CRIMIC Lines

The Bellen Lab has generated a collection of lines using a novel method called CRIMIC. Currently all lines are available to order from the Bellen Lab but are in the process of being deposited into the Bloomington Drosophila Stock Center.

Kanca O, Zirin J, Hu Y, Tepe B, Dutta D, Lin WW, Ma L, Ge M, Zuo Z, Liu LP, Levis RW, Perrimon N, Bellen HJ (2022) An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR mediated homologous recombination. eLife 11:e76077. PMCID: PMC9239680.

Kanca O, Zirin J, Garcia-Marques J, Knight S, Yang-Zhou D, Amador G, Chung HL, Zuo Z, Ma L, He Y, Lin WW, Fang Y, Ge M, Yamamoto S, Schulze KL, Hu Y, Spradling AC, Mohr SE, Perrimon N, Bellen HJ (2019) An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms. eLife 8:e51539. PMCID: PMC6855806.

Lee PT, Zirin J, Kanca O, Lin WW, Schulze KL, Li-Kroeger D, Tao R, Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N, Bellen HJ (2018) A gene-specific T2A-GAL4 library for Drosophila. eLife 7:e35574. [Abstract]

Images: CRIMIC lines are crossed to mCD8::RFP, and fixed third instar larvae or adult brains are imaged with a confocal microscope.

Step by step protocol for construct design

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