CRIMIC CR00506
CG6223
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
TTGCAGCATCCCAATGAGTTTCTGCG |
TGACCTTGTTCTCCCACTCGAAGTC |
Images
Construct Design Sequences
| sgRNA sequence |
TAATGGGCATGTCTAGCTAGAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| TGGATTGAAAGCGATGCCCT |
TGCCCATTATCTATTGTTCGGC |
TCAGTGCAAGCTGATGC |
CCGCTGGCACATTGATTACG |
| Amplicon 5' |
Amplicon 3' |
| TGGATTGAAAGCGATGCCCTTTCGCCTAAAGATGCACTACTCACTGGTCTGGTTGACGATCAGCACGTCCAGGACAATATCGTACTGATTGACATTGACGTAGGCCTCCGCATAGACGGGATCCGAGAAGCCAGTCAGTTGGGTGACCTTGTTGAGCTTGCTGTTCGGCGAGGCCACGTCGCTCAGCTGGGCGTTCTTGCTGCCGGCCAACGCCTGGTTCAGACTGGATTCGAACACATTCTCGCCCAGCTGATTGTCACGTCCGTTCGAGAGCTGTGCAAAGAGCACCGGATCGTCCGGCTGCACCTTGGCGGTGGCCTTCTGCTTCTCCTTCAACATGCGCTGGTCCTCGTCGTGCTGCGCATCCAGCATCTTGCCCAGAGCCTCGCGGCAGTACAGTGTGAAGACGGAGATAGCCTCCGGCGTGCGTTCGCTCAGCGTGCGCAGGCAGACGAAGATGCGATCGGTGTCATCGTTGGTTATGGGCTTGCTAGGGAAACCGGACTTGCCCAGGTGCAGTATCGAGCTCATGATCAGCATTACCTGCGTGGTCAGACGATTCTGGGCACGCGCCTCCGTCTCCAGCTCTGCGTAGCGCAGTGCCAGCTTGGTCAACGTGGCGGATAGAGCGGCGCCGATGAAGAAGTCGCCGTCCATCAAGTACTGACGCAATGGCGGACGTTTCTCCGCCTTGGCCACCCTGCAGAAAGCATTAACCAGCGTTAGAATCAGAACTGCAAACAACTATTTTGTAGGTGCTACGTATTAAGATGACTCATTGCCGAACAATAGATAATGGGCA |
TCAGTGCAAGCTGATGCAATGCCACAAGCCTCGAATGCCTCATTGGTGGCATGATATACGTACACGTAGCCATTACTTACGGGGCCAGACTGTAGGCACTCTGGGTGGCATAGGTTCCATCGGAGGTGACCTTGTTGCTGGCGTTTCCGGAGCCAGAGCCCTCTGCGGCGGATCCAGCTGCATTGCCGCCTGCGGATCCCTGCTGCTGCTTCTGCTCCTCGGTCTGGTCGCCGGCCAAGCGACGCTGCTCCGCCTCCACCATGGGCACTTCGCCCAGCGTCTGCTGAATGACGGCAATCACCTCCAGGATTTGCGAGCCCTCCACGTATTCACCCAAAATCCAAACGGCGGCGCGATGAATTTTGGAGGACTTAATCTGCGGGAAGGCCTCAATTAAATGCTCAATGATGAGGGCGCGCAGGGCGGGGAACTTTTGGATGGCTTCGCGTATGAAGATAAGCACATCGGCGGCGGCAAGTTCGTTGGTATCCGAGAGGAATTCAACCAGTACGGGTATAACATTGGCGGCCACATCGGGAAACTTGATGGAGCAGGTGTGCAGGGTGCGGACGAGCAGCTGCCTGTACTTGCCAGTGTCCTCGTGCTCCACATTGTGCGTCTTGGCCACCTCCTTCTTGAGGACCAGCACCATTTCGCCAATATTACGCGAATAGACCAAGTCCAGGGCCAGCGCGAGTGTTTTCCTGCGCACCTCGATGTCTGGTGCGGCCAGGACGCGCAGAACGTCCATCACCAGATCCTGCATCACCTTCTCCATGCCCTCGTGCTCCTTCATGGCCACCAGGCGATCGAGCACGATTAGCTTGACGTTGTTGTCGCTCTCCTTGACCACCAGTTCGATGTAGCAACTGGCCGCCGCCTTGATGGCCGTGGGCGCCAGCGAGAGCGTAATCAATGTGCCAGCGG |
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