MiMIC Cloning Vectors
The MiMIC constructs are available for order from the Drosophila Genomics Resource Center. DGRC barcode ordering numbers are shown below. See Venken et al. (2011) Nature Methods 8:737-743 [PDF] [Suppl] for more information about the technique.
| pMiLR-attP1-2-yellow-SA-EGFP |
1321 |
MiMIC or Mi{MIC} vector |
| pBS-KS-attB1-2 |
1322 |
General cloning and correction plasmid (Sequence) |
Gene Trap
| pBS-KS-attB1-2-GT-SA | 1323 | General gene trap plasmid (Sequence) |
| pBS-KS-attB1-2-GT-SA-mCherry-SV40 | 1324 | mCherry gene trap plasmid |
| pBS-KS-attB1-2-GT-SA-GAL4-Hsp70pA | 1325 | GAL4 gene trap plasmid |
| pBS-KS-attB1-2-GT-SA-Flpo-SV40 | 1326 | Flp gene trap plasmid |
| pBS-KS-attB1-2-GT-SA-QF-Hsp70 | 1296 | QF gene trap plasmid |
Phase 0
| pBS-KS-attB1-2-PT-SA-SD-0 | 1297 | General splice phase 0 protein trap plasmid (Sequence) |
| pBS-KS-attB1-2-PT-SA-SD-0-EGFP-FlAsH-StrepII-TEV-3xFlag | 1298 | Splice phase 0 EGFP-FlAsH-StrepII-TEV-3xFlag plasmid |
| pBS-KS-attB1-2-PT-SA-SD-0-mCherry | 1299 | Splice phase 0 mCherry plasmid |
| pBS-KS-attB1-2-PT-SA-SD-0-EBFP2-3xMyc | 1300 | Splice phase 0 EBFP2-3xMyc plasmid |
| pBS-KS-attB1-2-PT-SA-SD-0-TagRFP-T-3xHA | 1301 | Splice phase 0 TagRFP-3xHA plasmid |
| pBS-KS-attB1-2-PT-SA-SD-0-HRP-S | 1302 | Splice phase 0 HRP-S plasmid |
| pBS-KS-attB1-2-PT-SA-SD-0-Dendra-V5 | 1303 | Splice phase 0 Dendra-V5 plasmid |
| pBS-KS-attB1-2-PT-SA-SD-0-KillerRed-V5 | 1304 | Splice phase 0 KillerRed-V5 plasmid |
Phase 1
| pBS-KS-attB1-2-PT-SA-SD-1 | 1305 | General splice phase 1 protein trap plasmid (Sequence) |
| pBS-KS-attB1-2-PT-SA-SD-1-EGFP-FlAsH-StrepII-TEV-3xFlag | 1306 | Splice phase 1 EGFP-FlAsH-StrepII-TEV-3xFlag plasmid |
| pBS-KS-attB1-2-PT-SA-SD-1-mCherry | 1307 | Splice phase 1 mCherry plasmid |
| pBS-KS-attB1-2-PT-SA-SD-1-EBFP2-3xMyc | 1308 | Splice phase 1 EBFP2-3xMyc plasmid |
| pBS-KS-attB1-2-PT-SA-SD-1-TagRFP-T-3xHA | 1309 | Splice phase 1 TagRFP-3xHA plasmid |
| pBS-KS-attB1-2-PT-SA-SD-1-HRP-S | 1310 | Splice phase 1 HRP-S plasmid |
| pBS-KS-attB1-2-PT-SA-SD-1-Dendra-V5 | 1311 | Splice phase 1 Dendra-V5 plasmid |
| pBS-KS-attB1-2-PT-SA-SD-1-KillerRed-V5 | 1312 | Splice phase 1 KillerRed-V5 plasmid |
Phase 2
| pBS-KS-attB1-2-PT-SA-SD-2 | 1313 | General splice phase 2 protein trap plasmid (Sequence) |
| pBS-KS-attB1-2-PT-SA-SD-2-EGFP-FlAsH-StrepII-TEV-3xFlag | 1314 | Splice phase 2 EGFP-FlAsH-StrepII-TEV-3xFlag plasmid |
| pBS-KS-attB1-2-PT-SA-SD-2-mCherry | 1315 | Splice phase 2 mCherry plasmid |
| pBS-KS-attB1-2-PT-SA-SD-2-EBFP2-3xMyc | 1316 | Splice phase 2 EBFP2-3xMyc plasmid |
| pBS-KS-attB1-2-PT-SA-SD-2-TagRFP-T-3xHA | 1317 | Splice phase 2 TagRFP-3xHA plasmid |
| pBS-KS-attB1-2-PT-SA-SD-2-HRP-S | 1318 | Splice phase 2 HRP-S plasmid |
| pBS-KS-attB1-2-PT-SA-SD-2-Dendra-V5 | 1319 | Splice phase 2 Dendra-V5 plasmid |
| pBS-KS-attB1-2-PT-SA-SD-2-KillerRed-V5 | 1320 | Splice phase 2 KillerRed-V5 plasmid |
MI Line Search