CRIMIC CR01023

Gene DIP-epsilon

CG42368

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:6399387..6399365	26D1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGCTTGCTCTGCCAAACTTTGTAAC	CTATGGAAACGGTTTGGCAAAGAGCG

Images

Construct Design Sequences

sgRNA sequence	ACTAGAGGAGACCTCAGATGCGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCAATGGCGAACGACCAAGA	TCTAACCGAGATGCAGCAGC	AGGTCTCCTCTAGTCTGCCG	TTCCAGAGTCCTTGCTGTCG

Amplicon 5'	Amplicon 3'
TCAATGGCGAACGACCAAGACACTTTGATTCAAACTGTGGCCAAGGACAAAGGATTTCTGGAGGACGAAAGCCAAGGAGTCATGGCTGGAGGAACGGCTGCCCTCCACATTGCTGGCACTTAAGTGACAAAATCTATTCGCAAGAAAGTCATCTGGTTAGGGTATCTACTAGCCCATAGAGCTTTGCTCCGGTCACTCATGGTCGGTCATTTTATTCTTATACTTCGCCAGTGATGCCTTTGAGAAAAACTTTGCCACTTTTTCCATCATCGCCGACAGTTTTCCTTTTTTTTTTTTTTGTCTTTCTGTTTTTTCGGTAACAAAGCCGAAACTAAACTTTCCAGCGCTTTGCCAGACGGGTGGAAATTCTCGCTGGCCAGGGGCGTGAAAAGCGCTAAAAGCGTTTTAATGATTTTAAAGGAGTTAAACTTTTGTTTTTTTCAAAACACTTCTTATGCGCTTTCTTCATTGTCCGAAAAAATTGCTCTTCTTTTTGAACAATTGTTTTGGTTATACTGTGCTTTGATTTTATCGCTCATCCCGCGCGTTGACCGAGCATCATTCTAAGTTACATTCTAAGTTGTGCTCTTTTATATGAATAAATACCCTGTACTCCTAGAGGCATTGGGTATGCACACATCTATATCCAATAAAGATGTTGGACAGTCATCTGTATAAGAGAATTTTAAGTTCTTTCAGCTACATTACTTGGGATCAGAATTCCCAAAGTAAATACAAATGCGAAAAGTACTTAATGCAAGATTTCATGATTTCCATTTAAATCGAAGATATTGTCATAAAATTATCTCAGTGTATTTATCTGTCCTTCTATTTTGCTTCATTGTTTACTCAACTTGACAGATTAAGTAAATGCTTGAGGGGCCAATATAGAGCATCGCACATACATATATTTATCAACTGTGCATTTTCTATCTCAAAAGTGCGAACTTTAAGCTTTATGGAAAAAATAAGTTTGTCTGTAGCATTAAACCGAAGGAGGCACTCCATCAAACCCATGGAGGTTAATAGTGTTAATACATTTAGAGACATGTCGACCAGGTAAAAGGAGCAAGTATCTTAGTATCTGACATGGCTGCTGCATCTCGGTTAGA	AGGTCTCCTCTAGTCTGCCGTACGTTTGCAGTCCAAGCAGTGTAGAGTCCGAGAAAAGCGGGGAAAAGCTTATAAGGACAAGGAGTCTTGGGGCACGGGGTAAGTTCAATCAAGCAAATGTAATTCTGCCCCGACTGCCAATTGCAGGCAAGAGCACAAACAACAAAGATTGAATGGAGCACTGACAGTTTGACTGGGTGAATGAGTGACATAGGTATGTGTGTTCTTAAAGCCGCTTCTGTTGTTTTTGTCGTCACACATGATGTTTAGGCATGTTGGTTCGTATGCAGCCAAGAAAGAGGTGAATTTCAAAAGTGGACTATATAAACAGGGTGTAAGTAACTGCCAAGCCAAGCAAGCAAAAGTTGCACAGCAATACACACAACACGCTAGTCCCAATATTTGTCTATGTATGTACAACCCTTAACTATGATCAATGTCGCATGCTTACATCGTATTGGCAATAAAAGGGTATGCTGCATTCGTTGAATAATAGAGTGGAGTAGAAGGTAAGAGCAAGCCTACACTCGTATTTGTGCATACACACACACTATTGGTAAGCAAAGTCAGGAGCTACTCATGCAGAAATGCGCATAACTGCCTTGCAGACAGAGATTCACCTTCACACAAATGCAAAAGTTTAAGTAGCCCCAAGCCATTCTGGGGCGCCGTAGAATGAGCCCCTGCCATCGGACTCCATCAGGCACTAAGTACTGCTCTATTAACCAAGAACCAACAACAACAAAAACAAGAGCAGACGGTGTGGTCCCGACTGCCATTCGACAGCAAGGACTCTGGAA

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