CRIMIC CR00519
CG8271
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
short band |
CGCCAAAAAGGGCAACAAACGAAGAG |
GCCCCCTTAATATTTATGTGCTGTCGC |
Images
Construct Design Sequences
| sgRNA sequence |
GTCGAGGGTGGTATCTAGTCGGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| AAGACGAACCCGCAAAGGAT |
GGGAGGGAATCTAGTGGGGA |
TCCCCGACTAGATACCACCC |
TCGTGCCGCTTATGAACTGT |
| Amplicon 5' |
Amplicon 3' |
| AAGACGAACCCGCAAAGGATTCAGTGCCAAAGGAGCCGTTCCGGCAGAAGCAGCAGTAGCAAGAGAGTATTTATTTTGGGCCAACCGGTGAAGGCCCGCGAACGTCAGCTCTTCAGGACTTTAAGGACCCAAAGCAGAGGCAACCCAATGCATTTGGGTGCACAACAACAACGCCCCAAGCAACAACAACAACAGCGCGAGCAACAAGAGAAACATCAAAGGCGTTTCACGTTCAGTAAAGGACGGCAATGTCGTCCCTACAGCGTGACTGGCATTTACAGGTGAGTAGTACTACCCAGCATCCCATTATCCTTACTTTGGGAAGGTCAGGAACCATCCCCCTTGATTCCTCCTCGCTGCCTTTTCTGGGGAGTGGGCCAATGGCAATCGTATTGAGGTAGCTGGAGTTTGGGTTCCTCTGGTGAGCATGCGCACTGGACCAGAGAGTGTTTTACGAGTACGTAAACATTGCAAGCGACAGGAGAATGGGTCAACGTCACAAGTTGCATCCATCTTAGTCGCTTTAGAAGAACGGGGACAAGAAAGTAAACAATACATGCCCCGACTGCTGATGGAAAGACCGAAAACCACAAGCCAAAGTTCAGTCGTGGCATGGTGTTGACCTTTAATGACACCTATTAAATCGAATAGAAGTGTTCATTTCTACTAGTTTTGACACAAATGAAAAGTAACTTTTTATTATGCTTATAATTATACTTACACTTACAATCGTTGATTTTGTAAACACTTAAAACATGAATTTTGAGCCAAATTTAAGTAGGTGTTTACACTCGCATATGGGAGTACAGCATCCCTACACTTAAGCATCAATGTCCTGCTGATTGGGTGAACTAATTGGCCTAGGCCTGTGCAACCTAATATCTATTATTACGGAGGGCTAGCAATTACGGGTGGCTCAACAAGTTGTGCGTCAAGTCCTCCCCACTAGATTCCCTCCC |
TCCCCGACTAGATACCACCCTCGACCAGCCGACTTGAGGCCACCCCTTTCACCTTGACCGCTTATCGTTCAGGCCCACACACGAGCGCCAACTAACTTGGCCCGGGGGAAGGTTCTTCCGTTTCATTAAGCGCTGGCAAAGGCCTTGAAATGTTGGGAAATTGAAAACAACCAGCGATTGCAGAGCTGCCCCAGCGAAAGCTTCCTGACAGCTTAAGCCGGATTCCAGCTGACAGCAGAGGGTGGTCTTGGGGGTTCTTGGCGTCCAGGATATCGTATACCATCGCATGCCACGGCGACGTGGGCCTTTGACATTCGACAAGGTCACTGGCTCTAGGATGCTGTGACTCATGCTCTTGAGGGGGAGTTGCCAAATGCCAAGAGTTGGCCAACGCTCATTAGTCGTGTCAGATGTGTGACTGCATTTTGCACTCGTTCAAGAATATTGCATGCCATCTATTTGGCATATTTCTACCATCAATATGGCTGACAGTTCATAAGCGGCACGA |
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