| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_PM37_p1 | 3R:13008384..13011310 | 87D8-87D8 | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | AACTGAGTGCATGCCTGGAAGAAG | GGGTACCAGATCATCTGAAGATCG |
| sgRNA sequence | CATTGGCGAGAAATTGGAGTCGG |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|