CRIMIC CR00919
CG11206
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
AAGGAGAAAGCCGCTTCCGTGAACAA |
ATAGATGGTTTTTGGGGGACTTGGGG |
Images
Construct Design Sequences
| sgRNA sequence |
CTCTGCCCGTTCCACATCCCTGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| CCCGGTCACAAGTTCATCCA |
GGAACGGGCAGAGTACCTTT |
GAAACACCTAAGCCGCCACT |
TCTTGGCCTCCAAACGAGAC |
| Amplicon 5' |
Amplicon 3' |
| CCCGGTCACAAGTTCATCCATGTGAATGACTGATGATGAACCCAAATTGATTGCACACACACCGCCAGGATAATCGTTATTCACATACAAATTCCCCTGTGGATAGGCATTATGCCATCTTTTTCAGCTTTTTTGCTTATTGATTGTCAACACGGATTTATTTTACGGCCAAGGATTTAAATGAAAATTTAATTAGCACTTAATGCGCGGCATGAAATCATTATAAAAGGCAAAAAAAATGCATACAGGAATTGAGGTCGAATGCTCTGCACTGTTTACCAAATAAAAACTACCAAATTGAAGAGTTAACTTTTATTCAAGTCCCAAAACTAAAGTGGCTGAATCACCACCCCCGCTCCCCGTTAAATTTTCAGCGTTTAAGTTTCGAAAGCCCAACTCGACCGCAACCACTTTGGGATCCACCGACTTTTTCACCTTCTCCGGAATGACCCCGCCTATCTGCTTCGTTGACTTTTCAACTGCAGCACTAAAAGCGATTGGTCGGAGCTCCTGGACGGACAGACGGACAGATGGGGGGGCAGATGGGCAAATGGTGCTTGTATCTCCAACGAACGGACGAAGGCCATTACCATCCATTTGTCAGATTTTTGAAGTGTGCATCACTTTGCCATTCTGATATAATCTGATGCGTTGACCAAGCCAAAAGACCAAGAGCAAAAAAAGGTACTCTGCCCGTTCC |
GAAACACCTAAGCCGCCACTGCATCCTCGTCCTGCGGACCTAGTTGTTTTGTCCTGCTGTGGCTGGCTTGTGGCTCTTACCCTCGGTTGCCACTGGTAGCTTGCAATCTGCCTGGCCACAGGACAACATTTAATTTCTTGCCCCTTTTGTGCCGCCAAATACTTTAGCAATTGCATTAAAACAAAACAAATGGGATTCAATATTTTACGCAGCCGGCAGATGGAATTAAATCAGCGTGCAGGTTGAAAGGATTCAATCAGGAACGCTGATTTAATTCCTTTTTGGCCGGCTCCCATTTGGCATTCCCACAGTGGCCAGCATTTGTTCATTCATGCCGCAAAGATCCTTGCCCCATGCCACAGGCCTCTACCGTTTCTCCAACCAACCCCCCCTTGCCCAAGGAAACTCCTTTCTTTGCCCGCAGCAATCTGCTGATTGCTCCCTCCGCTCACACCCGCGCGCTGCGCGTTTACACATTACAAATGCATTTGCATACGCTGAGCGAAAAGCCAAATCCAAACAGTAATTAAAATAAAATTGTATTAAATCAAATTAAGAAAAAATGCTCCTTTGCTGATCGTTTAGGATCATTACCAGCAATAACTATCCCCAAAGGAATTATCTTTTTTAAGGTAAGTATTTTTTCCCAGTGTACAAGCACCCTTGCAACAGAAGCCGCCGCTATCCCCCGCACACTTACCGGCATTGAGTGCACTGCCACTGGAGGGGGCGTTGTGGGCGGGGCCGGCTGCCTGTGCCAAGTGACCAGCGGCAATTAAATTCGAGTTATTCGCATGGATCATGGCTGTGCCGGAGTTGCAAGTGCTGCTGATGCCGTTGCTGTTGCTGTTGCAGTGGCTGCTGCCGATGCCATTCGGCAATTGCTGCTGCTGCTGTTGCTGCTGAGGCACTCCTCCTGCGGCTTCTGCTGGCTTGCCAGGATACGCAGGACTCCGGTGCTGGTGGTGCTGTAACTGCTGCTGGAGCGGCTGATTATCAGCGGTCATGGGTGGGGTGAGGGTCTGGTTGCTGCCATTCGAACTGGACGGCGATGAGCTGCTCTCGTTGCTGGGCGTGAGGTCTCGTTTGGAGGCCAAGA |
Search Again
