CRIMIC CR01856
CG17029
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| long band |
OK |
GACACTGAGAGCTGAGATAGAGCATC |
CGTTGGAAACCTCAATGGGTTGTCCA |
Images
Construct Design Sequences
| sgRNA sequence |
ATGACATTATTCCTGCAAACAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| TCCTCTGCACATGAACTGCT |
CAGGAATAATGTCATGCACGGT |
CAGGTTTATCGGTGCACAGC |
CGTACACGGTGACCAAGTCA |
| Amplicon 5' |
Amplicon 3' |
| TCCTCTGCACATGAACTGCTCATATCAGCTCAGTTCATCCACTCACGAATTGTGAATTTATAACACACAAGATAGTCTCTAATAACCATCATACAACTTCAAGGAAACACACCTTTACATCTTTAAAATACGCAATGTATTATGAAAATATATCCTATGTATGATAGGTAATAGTAGAAGATAGATTGGGATATATCCTATATCCTATTGCAGTTATGTAGTATTAAATTCGTATATCAGAAAATATACTACAGGCTATGAAGATAAAAATGGTTTTCTTTAAATTCTATACCAATTTATACTCAATTTTATTAAAATAATAAAATTATCTTAAATAGTAAAAAGAATTTCCTATGCATTCATGGATTTTTAAATGTTAACAATAATAGTATTTTCTGATTCTTGGCTGACTAGTGTAGTAATATCACATCCATAAACTTGGAACAACGGTTCGTAAGCAACTCTATCGCGCTCTCCTAAATGTCCAAAGTTCTATTGCATCAATGAAAGCTTCGGCAGAAAGCTTTCCTCTGGCGTCGCCAACATTCCACAGCTGACTCGCGTCTATATAGTTCTGCTGCTCAGTCGGAAACCAACAGAAATAGCAATGAGTTCCGCGGTCAGCGAGTCCAAGCTCAAGGAGTACTACGATGTGGCCCTGAAACTGGTCCTCAAGTGCGGGCCTCTGATGCAGGAGGGCTATCAGAAGGCAAAGACGGAATACAAGGTCAAGGCGGACTTCTACGACCTGGTGACCGTGTACGATAAGCAAATCGAGGATATTTTGACCGAGGGATTGGTGGCCGCCTTTCCCGAATCCCTGATCATTGGCGAGGAGGAGTCGGCAGTTTCACAGCGCCAGGCGGAGCTGACTGATGCACCCACGTGGATCATAGATCCCATCGACGGCACCACAAACTTCATTCACCGCATCCCGCACTGCTGCATCTCCGTGGGCCTGGCCATCAACAAGGAGCTGGTCGTGGGCATTATCTACAATCCACCGGCGAATGAACTATTCTCCGCCTACAAGGGACACGGAGCCTATCTGAATGGCGAACCCATCCACACATCCAAAGTGACGACGGTAAGAGTGTGCTTATATAACCCGGGAGCAACAATACTATGTATAGCGACAAACCAGATAAACGAAATTTGACAAATAACCGTGCATGACATTATTCCTG |
CAGGTTTATCGGTGCACAGCAAAAAAAAAAAAAAAAGGTTGGCTAAACTTAAATTTGCATTTCGATAGGAATGTTTGAGAAATTTGATAAATGTGATAAAATAATTTATTATAACCGCTTTATAACAAAATTCCCATACAATTGTTATAACAACTTTTTGGAATGGAAAAATACAATCTGCTAACATTTTTTTCGGTGCACCGATATCATTCATTCGTATACCACCAAACAAAAATAGCTCTCCAAAATTAAGCGCATATACAATTACTCTCACAACTAATGTACCCCTTTCCAGATCAAGCAAGCAGTGATTGCCTACGAGATCTCCCTGATCCACGCCGCCGGAGTGAGGGACAAGAATGTGAAGCGACTGTACAAGATGGCCTCGAATGCCACGGGAACCAGGTGTTTCGGAAGTGCCGCCCTGACCCTGTGCTACGTGGCCACGGGCCAGTGCGATGCCTATCATGTGGAGGATCTGAAACCGTGGGACATTGCTGCCGGAGCCATCATTCTGACCGAAGCTGGCGGCACTGTGTGCCATACCAGCGGATCCAAATTCGATGTGATGAAACCGGATTGCGTGTGCGCCGCCACGCCGGAGTTGGCCAAGAATGTGATTAGTCTTATCGAAGAGGCCGGTCAGATTACAGAGTACACATTTAAGTAAAGACAGTTAAAGGCTCAGTATGCAAAGACAGTATCCACATTTATCACTTGTAAACTATTCTAATCGCGCAGATATTCCAGTACAATAAAACGACCTAAGTACATATAAGTATCGCAGAACTTTCAGGAGCTTGCGAAGAAAGCTTTACCATAATGTCAGCTGTGTTTCTAAATTAAGTATTAGTTTCTACTCTGATCAGTCGATAGACGAGCCATGAGTTACCGAATCGGCGAGGAAAAACTTGAAGTATATTACCAGGTATCCCTGGAACTGGTCAGGAAGTGCGGTCCGCTCTTTTTGGAGGGTTTCCAGAAACCGAAAACCGATTACGAAGTCAAGTCAGCGTTCTATGACTTGGTCACCGTGTACG |
Search Again
