CRIMIC CR00460
CG10492
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| short band |
OK |
ATTCGAGCTGCGATTTTTGGGCACGCT |
CGATCCACAGTTGTAGCAGGAGATCACT |
Images
Construct Design Sequences
| sgRNA sequence |
AATATTGCCGAGCTAAACAATGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| AAGACAGACACAGACGCCAG |
TGGATGCTAATGCTGATATTAGT |
GCATCCATTGTTTAGCTCGGC |
GGTCCTCGCTGGTGATATGG |
| Amplicon 5' |
Amplicon 3' |
| AAGACAGACACAGACGCCAGTGCTCCAGCATCCTCACCCGCCGTCCCCACATGACCTGGTCATTCAGCAGCAGCCACCACACGTGATCCACCAAATACCAGGACCGCAGCAGGCGCCCCCGCACCCGCAACATCCTCAAATGGTGCAGGTACTGCAGACGCCGCAGGGCAACATACCCATGATCTTTCCCCAGCACTTTCAAAAGGTAAATCTTTAAATAGAAAACCACTAAAAGTTTATTAACTTAATCTGTTCATCTACAGCTTCTGCCCAGCTCTGATGGAACGCACCAGATCAGTGTCGATGGCATGCCGCACCTGATGCAGCCTAACATCACCATTCCAGCGGACACTAGTGCCATCATCAGTCACCAGAACACGGGATGGACGATGCGTCACTACGTGCCACAACCGCCCCACCAACAGCCCGCTCTGCAGCAACAGCAGCCGCAGAGTCTGGACCATCAAGTGCCACCTGCCAGCTCGCCGATGCTGAGTCAGCAATCCTCGCCATCGAGTAGTCGCACCACGAGTCCTCAGCGGGACCGCTCCCTTATTAACGTTCCACTGCAGGCGCAGCAGCAACCACATCAGCAGCAGCAGATGAGGGGCATGTCCCAGCGAATGGCGGATCAGAAGAACACGCGGCGTCCCTCGGCGGAGACTACCCCGCCGCCAACCTCAATTGGCGGCGAGCAGCAGCACGATGTAATTATTAAACAACTAAATAATTTGTTTATTTATTTGAAGCAAACTCAAGATTTAAATTTAAAGGAAATTAACTAATATCAGCATTAGCATCCA |
GCATCCATTGTTTAGCTCGGCAATATTTAGTAGTCTCCATATAATCACCATAATCATATCGCTTATACGACAAATAACTTACCTTTACAGAGTGTCAACGAAGGCGGAAGCAGTAATTCCAGCGGAACCAGTCTGCACAGACTCATCCGCAACGGTTTTCCGCGTCTAGGACACAATCATCGAGTCAAGGCAAATACCTTCATACCACAGCACCAGCAACAACAGCAGCAGCATCACTTTCAGAACCCCAACTACAACATGAACGTTATGATGCAGAGTATGAATATAAACGACGATGGCAGTGGATCGATGATGAATTCCATGAACTCCACCAAAAGTGCAGCTACCACGGGTAGTGAATCGGGCAGCTCGAATGGATCCTGTGGTGACCTGTCGCCCTCTGAGACGCCAACGCCCACGCAGCTCTCACTGCCACTGCACAATTCGGTGGATGCGGGAATGGGCATGATCACGACAGTACTGCCCACCGGTCTGGGAAACTATCAGCCGAAGCCACACCACTTCAGCTACAAGCAGCAGCAGCAGCAAATGAATCTGCAGCAGCGGCTAAACGGACGCAATGGGATGGATAAGACCTATCAGCAGGTTTACACAACGGGCACGCCAAGCATCGTCAGCGATGCACAGACCATCAGCAGCGCCAGCCTGCAGCAGCAGCAGCCCCAGACCCTTCTCATCAGCCAGTCTCCGGTGAGCACACCCACGACAACGGTGCTCCTGCAGCCGCAACAACCGCCGCCCACAACCTACAACACAAGCTATCCATATCACCAGCGAGGACC |
Search Again
