CRIMIC CR01567
CG5935
Information
| Donor |
Location |
Cytology |
FBti |
Stock number |
| pM37-p1 |
2R:.. |
|
|
Order from Bellen Lab
|
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| short band |
OK |
GCCAAAAGTGAGCAACGAAAAGGGCA |
GTCAGAGTCGGAATTGGCAGAAGGAT |
Images
Construct Design Sequences
| sgRNA sequence |
CTCCCAAACGAAATTAGCATTGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GAAAAAGCGCCGGAAAGTGT |
GAGCGCAAGGGAACATGAA |
ACGTCCCAAGGTTGAAGACATT |
CCGCTAGACTTTCCGCTCTT |
| Amplicon 5' |
Amplicon 3' |
| GAAAAAGCGCCGGAAAGTGTGTGCCAGCGAGGTGAAATGAAAACGGCGGGATTGCTAACGTATTTTGTGTGTAAATAGAGCGCAACAAATTATCCGCGAGGCTAACTGCCCGACTATCGGTTCTCTCCCGCCGTTCGGTTGTGTGTATAAACATGGCGTCGTTGTGGCTCGAGATGCCGATGCGAGTGGGTGCGAGCGGGATGACTAGTGTGTGTGTGTGCGCCTCATAGATTTGCAATGTTGTTTTTTCTATCGCCTCTCTCATTTCTTGCTCCCTCTCTCACTCGCTTGCTCGCTCGGCGAAGAAGACACGCATACACAAACTCGCAATCGCCGAAGACCTAACTCACACTCAAATTGCGATTCGATTAAAAAAGAAATAGGCGCACCGAAACCCATCGTATTGGCCGCCAAAAAATTAGCAAGTGTAATCCAATTCTGACTTTGTATCTTTCCATGCCATCGCCGCACGAAAAGGATGCTAACAAAGCGGCAGAAACGGCAGCCAAAGAAAATGCCGCCGATAAGGTAAGCGATGTGGAAAATGCATCGGTTACTGCGGGGGTGGCGAAAGCCGTGGGGGCACAGCCGGAAAGGGGCTCCAAAGATGCAGCCGAATCCCCCGCCGCTGTCGACGCCTCTGCCTCTGCCGCCACTGATGACGTCGCTGATAAAAAAGCCAAAGGAGACTCAACGGCTGTTTCAAATACCGAATCGGATGCAGGTAAATAAAAAATTAAAAGGCAAAGCCAGGCGAACACACAATCTAACTAACTGTGTGTGTGAAGAACGAGAGAGAAAAACAAAAATCAAAGTGAATTTACCGCGCATTTTAAAAAACGCGCCGAACTATTTCGCTCTCTCTTCGGGAAAGTTCTTTTCCTCTCTCGTCCTCTTGTTCGCTTTGGTCTACTTATTGGGAGAGTTCCGATGTGGCGCTGCGGTCTAAAGCGTCGCTCCTTGTGTCTCTCTTGTTTACCGCGCTATGCTGATGTTGGCATGTGGTTCGATCCCCCTCCGTGTCGATGTTTACAAAACCATTATTAGAGTTTGATGATTGAGTTCTCTTAACTTTCCTTCCTCCTTCCTTCCTTGGCTTTTGTTCATGCTAAATCCTTTAAATGGGTTCTGCGTAGTTTAATGCCGAGGTACAGCAAAACTTCAATATTCATGTTCCCTTGCGCTC |
ACGTCCCAAGGTTGAAGACATTTTATTATTTTAACATCTTTTTTATTTTATTACATTTGAACTCTTACAAATAATAATAATTACAATTAATATTATAGCTGCAGCGGACAAAAAGGAGAAATCCCCCTCGCCGGTAATAAAGAAGTCCAACAATAAGGATGCTAAAAAGGAGGACAACTCCGAAAAGGACGAGGAGAACTCGGAAGACGGCGATGAGCCAGAAGATGAGGCTGATGAGAAAGCTAGCGATGAAGAGAGCGAGAAGAAGAAACCGAAATTAGATGCAGAGGACAAGATAAAGGATGCCACTGATGAGTCCAAGCCAAAATCGGGAGCCGATAAGCCCAAGAAACCTGAGCCCAAGGCCAAGAATGGCAAGGTGGCTAAGGAGGAGGACGACGACGAAGAGGACGAGGATGATGAGGATGCCGAAGATGACGATGGAGACGAGAACGATGGCCTGGACAAGAACAACGAGGTGGCCGAGGATGATGAGAATGTCGTCGCTCTCGCCGAGATTGATCGCATTAATGAGAATATCAACAAGACTCGTGTAGATGGTCTGCAAACATTGCATGCAGTAAGTTAGCTGAGAAACATTTTTGCCAACAACTTGTCAAAAACTAATTTCACACCTCATTTATAGATCTGCTTTGGCGCCCAAGGCAAGAACAATGTGGTCAAGAAGAACTTGCGATCCTTTGCCGGTTTCGAGTTTGCCAAGGATTCAGCGGAGTACAACAAAAAGCTGGAGGCCATCAAAAAGGTGGATAATAAGGGCCTGCGCAGCATCTGCGAGATCCTTACCCTCGATCGCAAGGGCAGCAAGAACGAGACTGTCCTTCGAGTGCTCAAATTCCTAATGGAACCGGACGAGTCGCTTTGCTTGGAGCAGGGTGATGAGGAGGAGGAGGAAGATGCCGAGGACGAGGATCTGGATGAAGATGAGGAGGACCCGCCCAGTGAAGAGGACAAGAAGCGCAAGAGCGGAAAGTCTAGCGG |
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