CRIMIC CR00927
CG30460
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
TTTGGACGCATCCACAGCAACAAGCA |
GATTTCTCTGCATGGGCACTAGGCTA |
Images
Construct Design Sequences
| sgRNA sequence |
CCTAACCCCCGATGCTCTACTGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| TCAACCCATACCTTTGCGCT |
CCGATGCTCTACTGGCGTT |
GCATCGGGGGTTAGGATAAC |
CGTCTAACTCGACACCGGAG |
| Amplicon 5' |
Amplicon 3' |
| TCAACCCATACCTTTGCGCTTGATTAACCCATTGAGCTCGTCAAATGTCATGGTTACTTGGGGAAGGCAGATTTGCTTTTCGACCGCATCTAGTTTATCCGTCTTGGGCCCTCTCTTGCGTCGTCTAGCTGGCTGGAGAACTGGCGCTTCCACGCAATCGAGTTTGGCCAACTGGAGAGCATCTCCACTGCCGTTCCGCCTTAGTTGTTCCATGGTCTCGTCAATTTCTATGCAGGATTCCCGCTGAGTGCGCTTGGAACGGCGCAGTGCAGATCGTTCCCTGGGCGGCGGCGATGATGGTGGATCCTCATTGAACAGGTTCACTTGGTTCTGGGTAACCGTGGAGTTTGCTTTGGCACGACTTCCTCCTCGCGAGCTGGAGACTTGAGCTTGAGTGCTGAGGATCATTTTGGGGATGAGTGAAGATCGCTGATCGAAGAACTAACTACGGTGTGTTGAGACTACACGACGGTGGCAATAATTGCTATCAAAAGGTTACGATTACGATTACGCTTTACGGGATTAATCAAGGACGACTAAGATAACGCCAGTAGAGCATCGG |
GCATCGGGGGTTAGGATAACCTATCTAAGAGAAATCTATGGATTTCGGAAAACCCCTACTCACTTATCACTGTGCACGGACGACGTCATCGAATGGGACATCAGGTTTGGTTTGGGTGTTGTCATCTCACCCGCTTCCAGAACCTCCTCGTGGTTACCGGAGTACTTATCCCTGGCGAGGGATCTCTTGGTCCTGTCAAACATCGCGGTCAATTATATAATAGCAACAAAATAAATTGAAACGAGAAAGAAAACAAAAACGTGTGTTAAGTAGATAGATCACAGGGTGTCAACTAAATCCAGGCTTACTTCAGATTGGGCGGCCTGTACTGCTTCCTGGCCTCCAGGCGCTCCTTGGCTTTGCTGGTCTCCTGGCTGTTGGTGGACTTCATGGTGGCCTGAATGGCCTTTTCCTTACACCATTCTACGTGGCGATCAAATGCCTTGGGATTAAAGGTACGATCGCAGTGGGGACAACGATCACAGGGAGGTGCCTTGATCCGCTTGGCCAGCGATCGTTCGCGGACAAGAGGAGCAGCTGGAGCAGCTGGAGCAGCCGGAGCAGCTGGAGCAGTCGAAGCAGCGGGTGCCATAGCTGGAGCCTCCGGTGTCGAGTTAGACG |
Search Again
