CRIMIC CR01562

Gene gus

CG2944

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:5116990..5128321	41D3-41D3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	OK	GTACCGTATAGAGTGCAGTCAAG	GCAAGATGAGATGTCATTCCGTG

Images

Construct Design Sequences

sgRNA sequence	ACTGTTGACGGTTCCTCATCCGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCTCCAAGTTTGTGCGACC	CGGGTAGCATTGTACCCCTC	ATCGACAGTATCCGGTCAGC	TGCACAAGTGAGTAGGTCGA

Amplicon 5'	Amplicon 3'
TCCTCCAAGTTTGTGCGACCAATTTTTTGACGAATTGTTCTTCGGCAAAGATCCATTAGGGGCAAGGGTTCGGCTTAAAAGATACAAGTAAGAAATTGTTTGTTTTTTTGTAAAAATATATATAATTTTTTAATAAGTGTCATGCGAACTCTAAATAATTAATGTACTTTTCGCAATGTAATCATGTAATCCACAATTTATTTAAAACTTAAGAGAGTGCTATAGTCGAGTGCCCCGACTATCAGATACCCATTACTCAGCTAATGGAAAGCCGAATGTGAAAGTTTAACAGTTTTCTGACATTGGGGCATAAAATGAGAAAAAAATTAAAAATAGTTCAAAAACGTGGGCGTGAGCCTTCTGGGCGTATGTAGAAACCGATAGAAATTGGTAAGACAACCAATAAAACGAAGAAAAATTAAAATATTTTTCAAAAGTGTGGGTGTGACAGTTTTCGGCGATATGTGGGCATTAGAGTGGGCGTGGCAAAACGTTTTTGAGAACTCAATAGTAAATTATAAGACAAATAAGAAAAGGTCGGCTTGTATACGTGGCAACATCAGTTAATATCAGTTCCGCTGCGTCTAGGTTTCTGGAATCTGCATGCCTACTCTGCCTACACCTTGGAGTTGGAGTCACACTTACCCAAAATGTGAACTTTAAAGGGAAACCACAAATCACGATTGCAGACTCTAAGGTGCTATAAACTTCATGTTAACGCCACAGCGCTCATACGAAAAGTAACATCATTCACAACATTCACAACCTCCAAAAGCAAGTCAGAGGGGTACAATGCTACCCG	ATCGACAGTATCCGGTCAGCCGAGCAACGCATTCCGCATCGGCAAAATCAATATGAAACGGTCAGCCATGAGTCACGAAGCTCCAACTCGCACAAAATGGCACGAGGCGCTGAGAAAGCGCTCTGCCAGGATCTCTGCCCTCAACGGAAGACTTCGCGGGATAAATATGTTAACTCTATAAGTTAGTACGTTCGAAACCAAAAACAAGAAAAAGTTTGCTCAATAAATAACCCTTTTATTATAATAACCTACATGGTTTAATTTATATCTTAAGTAATGGAAATTGCGATAGGTTGACTCTTGAAACATGACACAATTTGGGAAGCGCTGGCAATTAAAACCGAAGTTCTGGAAGACTACTTGAATATTTATATGTGTATGTAGCCCTCTCATCCATACTTGTGGATGCGCACGCTATTCCACCGAACTTAAGAACAAATGCTATATCTCCCATCTTCAAAGCAAGTACAGAAATGTCGTCTTTCAACTCTGTATAGAAACATATCGTAAGACACTCAGAATTTCCTGGAAAAATCTTGTTTCCTCAAGGAGGTCCGAACACGGACTATCCCAAACGCACGCTAAATAAGATAACGAGGTTATTCTACTACTAAACAACACGGTCTTGACCGAACTGGGCGTGACCGGTTTGGGCGTTTTGTAGGTGAAAGGAGGGGAGAGGCTACAGTGTTTTTGGTATACGAATAAAAAATGGCAACACAAACAATAAATCGATAAAAACAAGAGAGAATGCTATAGTAGAGTTCCCCGACTATCAGATACCCGTTTTTAAGCTAGTGTGAGAATGGGAACGAGAAATTTCATCGGTTTTTCTGGGATATCGATAGATATTAGGCAGTAAAATGAGAAAACAAATTTCAAATATGTGTGTATAAAAAGTTTTTTGGCACATCTATAGAAAATTACATTAGTTGCGCTGCGTATTTGTCTCTAGAATCTGTATGCTCAAACTCAACCTTCTAGCTTTTATAATACGTATAATTTATATGGTCAAGAAATGTAGTATACCCTTTTACTCTACAAGTAACGGGTATAAAAATAAATACTACTACTATGCATGTTTACGTCCATTGGCTTTGAAACTATCCGATATTACTTTTCTTACACTAAGCACAATTCATCTTTTAATATTGGTCTTGCAAAAAAACCTTGCAAAATCGACCTACTCACTTGTGCA

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