CRIMIC CR00576

Gene vtd

CG17436

Information

Donor Location Cytology FBti Stock number
pM37-p1 3L:27140907..27140929 80F Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK TTCCGACCCCAGCTTCTTTGAGCTTA TAGACTCGGACTTCGGGAAACTACAG

Images

CR00576-1.jpg

Construct Design Sequences

sgRNA sequence AATTGAGACCTACGAAGTTATGG
sgRNA2 sequence
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CCCATGCTGATATGCCTGGT TTGAGCTGACACACAATGGC TGGCGAATCTGCAAGGTGTA TGTTCGCCCACTAAGACCAC
Amplicon 5' Amplicon 3'
CCCATGCTGATATGCCTGGTTCTCGATTGGATGGTGATGGGTTTGGAGATTCTTTTGGACGTAAGTATAATCAATATCATCAATTACAAATGATATTAGCTGGATATATATATGGATAAACCGCGTAAAAGTAGAAATCTAAGAAATATATCCTCAAATATATATCAGTCGAAAGTATCCTTAAAAAAATTAATTCAGTTGTAAAATACTCCGTCATAAAAGTTGCCTTAGTCTAGTAATACACTTTCTTCAGTCTGTTGTTTTTTTCTTATTTGTCATCTGTCATCCATATGGATTTGAATATCTTAAATGCCTCTATAAAATAATTTTTAATCTTATGATCTGATTTATATACTAATGATGCGGTAATGAGCATTAGGAGTTGTGATACAAAAATCATTTGGTACAACCATATTTGGTATGGTGGTATACAGTTACAAAGTATTTTTTAAAATTAATTTGTAACTTAGGCGGACGTAAGTATCTTAAGGTGAAAATTTTCTTTCTTATTACATTTTTTTTTTCTTATTACATTAATGCATAAATATTGTAAAGAGCTTGTTTTCCTAGTTCTGCCACTACGAGTTGAAATGGCTGGCTCAGAGGGTTTTGTCTGTTTGCCCAAATTTATGATTTGTGGGTGATTATGATTATGATTTATAATTCTTCAGCTGTATTGTTTGAATACTGGCTCTGGACTTCTACTGCCATTGTGTGTCAGCTCAA TGGCGAATCTGCAAGGTGTATCGTCCGTTAGGTCGAACCAAAGCTCTTACCAGAACCACCTTTGCGAACACCCGAAAATGCACGTATGTTAGAACCACTCCTGAAACAGGACACCAGATTCCACGATCCCTTCTGACCACGCTATGCCCACGTCGTCTCCCAACGGTCCGTCCTGAATAAGCCCTAGGATATTATGGTGGCTTGGCCGCTAGGTGTGTCGTTCCTTAGGTCGAACCAGGGATCTTCCCAGAACCACCTTCGTAGACAGCCAAGAGCACATCTGTTGGGACCGTTCCTCTCACAGGACACTAACCAATGCCTTCACAGTCCCTTCTGACCGCGCCAGGTCCTTGTCCTCTTCCGTGTTCTTGCTGAGCGTTGGTCTGCTCCCTCACTTGCAGTGATCAACCTCGTACTACCGTTCCTAATGCTCACCTTGTGATCTCACTCATTGTCCTTTTTTCTCGGTCTTCTACACCAAATCGAAAGGTCTAGGTGTACATTCTGCGCTTTTCTTTTCTTCTTCTTTTTCTTTCTTCTTGGCTATGAGATGATACTGAGTGTGTGGTCTTAGTGGGCGAACA

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