CRIMIC CR01535

Gene Tsp5D

CG4690

Information

Donor Location Cytology FBti Stock number
pM37-p0 X:6069131..6074857 5D3 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK AGGTCGGACGATGACAGCTCAAGATA GTTTCAATGAGCACGTGGAACTGG

Images

CR01535-5-1.jpg

Construct Design Sequences

sgRNA sequence CAGCTCGACGTAGACTTTGGCGG
sgRNA2 sequence
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CCAACCAGGAACTCGCTCAT CGGCGTGTTTACCCTTAGCT ACGTCGAGCTGGAAACTGAA GACAAATGGACGGCCCTGA
Amplicon 5' Amplicon 3'
CCAACCAGGAACTCGCTCATGAACAGCATTACAATCAGCATAAAGTACTGCGGGTAGATATGATATTGAATGGCTTTGCACAGAAAGAAAATGGTCAAAAAAAAGTACATAGATCACAAATTCGACATGCATGTATGCCATGAGCTAAATCCTCTCCGTTGACCCTGATTTACCCCCGATTTACTCACCAATACCAGGAGACAGCGGGATTGCACCCAGGCGCCGCAGCAGCCAAAGAAGCTGACCACAAATCCGGTGCCCCCGATGCCCATGAAGATGGTGTCCGCACTCAGACCTGCGTGCTGGGGCAGCAAAGTGGCGTATCCCGCGTAGCTCAGTCGCAACCAGAGTCCCGCTCCTAAAAACGCACAGCTACATAACTGCAATGGGTCAGGTTGGGAATTGGAAATTGGAAAATAGAAATTAGAAAGGCACTGATTATCCAGCAGAATGCTCGAAAAGTGAGAGGTGGTAGCTGCATACCTAAAGGACTCAAAAAGCTTAATATTTGATATAAATCAACGAGTCAATTATTCCAGACAGTGTCACCTGGACACAGACCTTGATTTATTGCTCTTTGCTGCACGAATAGCTGAAGGATCTATCATTCAGAGGCGAAATCAGATAATTCCCCCGATTGACGGCCAAATGGAGAGCTTTCGATTGCAAACTGTTGCATGTTCACAACGGCCTGACATCTGACATTTTCAGGGACTTCGGGGGAAACCCAGTGGCAAGCAAAAACAAAAGCGGACGGAAGCTGCAGCTGCAGCTCCAAAAAGCTAAGGGTAAACACGCCG ACGTCGAGCTGGAAACTGAAACTGTGCTGAAAGCAAAGCCAAAAGCAAACGCAAAAGTCAATGACAGGCGCTCAGACAATGCAACATGCTGTCACAAAGACACTTCCGTCGCAGACGACGGCAAAGTTGTGGCTCTGATTAGCCGCCCTACGTACTGATTTTGATTTTGCCTGCGATTCTGATGCCGCGCATATCCGTTCTGGGGGAAATGGAGACGGCAGTCAGAATATATGTATCTTTCCAGAAATGTTTACTTATAGGCATAGAGGTGTGTATTTCAAGTCAACTTATTAGTAATTAGTAGGATTTTTTACTTAAATAACTTGCCCATAGAATGATGTTGAGCCAGCAGAATGTGCGGCGTATGCAAGTGTAGCCGGCATTGCCCATCGCGGGATCTGAAACGAAAAAAAAAACCATCAGGTAGAGCTCGAAACCTTTAATAGTCCTTTATATTTAGTGTAATTCCATCCTAAAAAGTATATCCTGTGGAAAAACCAGACCTTGCATCCATTAAACATCCATTTTGAAGCAGCAATTGCAATTCTGTTCGTTTATATATCAAATATAGTTTACTGTTATTATTATGTGTATTATTACTATGTTTTTTCATTGGCATTTCCTTTTGTCAGCGCCATGTAACTGGAGTACCCACATGTGTGTATGTGTGTGTGTGTGCCAGCGGGGGCGATTCGTTAGGCAATTGCAGCTGCAACTGCTGCACAAATGCAGTAATCCACAAATGTCCACACATGGGTAGACACTCGCTTATGCAAATATTTTGTATGATTGAATGAAAACAATAACTAAATAAAAAAAAATAACAAAATTAACAACAGCAGCGACCGCAATGACCATGGTCAACGAAGCAGATGAGCAAATAAATGGGTGGGTGTCCTCATCTGTATATTTAAACTATGGATGGCAAAAAGCATTCAGGGCCGTCCATTTGTC

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