CRIMIC CR00030

Gene Dhap-at

CG4625

Information

Donor Location Cytology FBti Stock number
pM14 3R:25488378..25488425 96E2 FBti0200720 81135

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK short band GGCTGGGAAACAGCACAACTGCTC TTGTTACTGAACTCCAGCTCCAAAGGGAT

Images

Construct Design Sequences

sgRNA sequence GAAAAGTGGCGTAAAATTAGCGG
sgRNA2 sequence
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CACATCCGCCTTAATGCTGC GTGACTGTCAACGGATTTGC AAAGTGGCGTAAAATTAGCGGA CGCCTTCACCTTTAACACGC
Amplicon 5' Amplicon 3'
CACATCCGCCTTAATGCTGCTCGGAGTCGTGCTAACATGGGCGCCCAGCTGCTCGAAGATTCGCTTCAGATGCAGAACACCTCGTGCTAGCTCGTCCAGAGTGACACTCCTTTGGGCATACAATTGACTGGCGTAGTAGAGGCTAAGCAGATTGAACGTGGAGATCACAATGCGACGCTGCTGCTGGTAGATAATCTCATTGGCCAGCTGTTTGACTAGTTCCACCTCCTGGCGGTTCAGCTTCTGCAAGTGACCACCCACGCCTGCTCGCTGCATTCGCTGCGCGCTGTCGTGTCCGAAAAACTCCTTGACCGAGATGGGCTCTCCGAAGTCGAGGAACATCTTGCCAAATCGCTCGTCGATGATTTTTAGGGCCTTGAAAAAGCCCTTTGTCGACTCCTTGGGCTTGGGCACACCCAGCAGCTCATAGACGAACAGTTGCTCCTCCAGGACGCGTTCGTAGGCCACACTCACTGGCACAATCATCACATCCGGCACTTCGCCCGTGAAATATGGCAACAGAGCCATCGAGAGTAGTCCAATTTTGGGAACCAGAGCTTTGAAATTCCTGGAGCGCGTGCCCTCAATGAAGAACTCCACGCCGATGTGGTAGTTTGCCACCAGCGCGTACATATACTCCCGGAAAATATCCCAGTATAGCTCATCGTTGGAGAAGCTGCGGCGCATGAAGAACGCTCCTGTTTTCCTCAACATGGTGCCCATTCCGAACATGGAGTGGAAATCTATAAAAATTAAGTAGACAACAATTAATATTATTGGGCAATTAAGAATATTAACGACTTTTGTTTAGAAACAGCGTACAAAAATTAGCAAATCCGTTGACAGTCAC AAAGTGGCGTAAAATTAGCGGAAAATATAAGCTCGCTTAATGTATTTAACACATTTTCCGCCAGATTGTAATTTGTTTATGGTTTTTGGCGAAACATACCCATGCCGGCTGCTATTCCTGGTATCTCGATGTCGTAGTAATAGCAGATGTAGGACATCAGGATGAAGTCCATGTAGCTGCGATGACTTGGCAGATAGAGCACCGGGCACTTGCCCATATCCTTGCGCACATTGGCCATGCTAGCCGAATTGACATAGAATCCATCGCAGATCCGCTTGCCGATCGCCGTAATGGCAATGCCGCACCACCGGATGATGGCCATATTCCGATCGAGGCCGATTTCATCAATGATCGCCCGGGCCTGACGCTCCATCTGCTTGAGCGGCGTGCCCGACTCCTTGGCATAGTGCTCCAGAATGGATCGCAGCTTCTCGCTCCTAAGGACGTGCTGCTTGAGCTTTTGCGGATTGAGATACTTTTCGAATTCGTATGCCACCTGCGGATTGAACTCCCTGGTCATGGAGGCCTCGTTGCCCGGCGCTATGATGTTCTTGAAGTTCCTCATATATTCGGCCGCACTGGGCGTCTTCTGCTCCTCCTGTTCGCTGGATTCACCTTCTACGGTGCCGGATGACGTCGACTGCCTCACGGGATTTCTATCCATCGCGCTGCGCTTCAACTGTGGAAATGGAAAAAAAAAAACGTGCGTCGGTCCGATGAGAATCTCTGGAGGTAGCTGACTGAAAGAAAATATTTGACGAATCAACTGTCAAGCAGGCGGCCCCACTTAACAGACATGACACACAAACACACTGCCATAAACTCGCTGTTTGCTGAGATATCGCAGGCACTGGGAGAAAATGTGTGCAACGAAATGTCGGAGTAAAAGATATATATATTTTTAATTTGTTAAATGTGCCATTTAAGTGTATATTAACTTTTTAAACACTTATTAAAAATTAATTTAACTTTCAAATTTGTATAATTTATAAAAAGAATTTAATTTAAATTTTGAAATATGCAGTGCATATCGAGTTTTATGTCTGATAGGTTATAGGTTCATTGCGCCAAGTGATCGTTTGTGTGAAGAGTGCCTTGCTATCCCAAAGGTTGATTATCTGACAGATTCATAAGCAGTTACGGCAATCAGCGGCGAGTGGCGAATGTTGCGTGTTAAAGGTGAAGGCG

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