| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_SA-GFP-Stop_p | 2:12670483..12679593 | 49B12-49B1 | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | L-F CCCCAGTAGAATTTGAATCAGTTGC L-R Aacttgtggccgttcacgtc | R-R GCGATGGCTGTGAATAGAAATACAAG R-F cgcatgattatctttaacgtacgtcac |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|