CRIMIC CR02110
CG5447
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
long band |
AGTTTGAGCTGGTTGTTTCGGAGGAC |
TTGTGGTGTTGGTGGATGTGGTGGAA |
Images
Construct Design Sequences
| sgRNA sequence |
TGTTATACGAGTTTAAAATAAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| TGGAACGATAGGGATGGGGT |
AACTCGTATAACAGATAGCACGA |
ATAAGGCAGTGGCACTTCCA |
TTTGCACGCCACATTTTGCT |
| Amplicon 5' |
Amplicon 3' |
| TGGAACGATAGGGATGGGGTAACACTATTTTGGTATAAAAGCCAACTCGCCATCTGGTAACACTTGGAGTTTGCAAACATTTATCTCTCGACCAATTTAAGGAATTTGAGGCAATTAGGGCATTCCTGTGAGCTTTTCCAGGCCAACTGCGATTACTTCAAGCCAAGACAAACAGGTGGGTAACCAAGCAGGCCGTGCCCCACCGACCAGACCAGCAAAACACCCCCATTTCGGGCGGTGCTGGAAAAAGCAAGGCCAGCTGTCCATTTGGGATTTGGACACTCCACTCTGAGCATCAAGATGATTGCCCTTAACCAAATGCATCGGGCGCTGCAGAGCGAGGCCATTAATGCCATTATCCGCCTTTTCAGCGACAACATGTCCCAAGGCGGTGGCACTACTCCAGCGGGCGGTAATCCCACAGCGCTCCAGGCGACCGGAGGGGGCGTGGTCTTCAGCAAGGTCTTCATCCAGCGCGATTACAGCGAGGGCACCTCCGTCAAGTTCCATACACGGCTGCCCGCTGAGCTGGAGGGCATGGTGACTATATAATACTCTATATTATCTGTGGGATTATCATCCGAACTCATATCGATTCTCGTGCAGATTGAAAGACACGTCTTCGAGGCCACCATCAATAGGCTAAACGAGTTCTACGCCGAGGCGGAGGAGGGATCCTGCGGCACCTATTGCGAGGGCTGCATTGGCTGCATCACGGCCTACTTGATCTATATGTGCTCCGAAACGCACTATGAAAAGGTGCGTCATTTGGGTTTGGATTACACACATGTGGGTCAATGCAATAGAGTCTAGTTCCACTAGGGAACTATTAATAGGGATTAGAGTCAACAATTTCACAAATGTCGATATGCGATCGTATAAGTTATCAAAGTTATTCTCGTTAATGTCCTTGTACTCAAGGTTTTCATATCTGTTTAAGGTATAAAGGTCAGAGAACTTGATAAATCAAGTAGTAGTATTACTTGCTAATTAGTGATCGTGCTATCTGTTATACGAGTT |
ATAAGGCAGTGGCACTTCCAGCTCTATTGCCTGCACATACTAATGCTCTTTCGCCCCCACAGACCCTTCGCAAGATATCCAAATTTGTGGCTTCCCAAAATGAACGCATTTACAACACCAAGGGACTGCAGCTGATCGATCCCACTTATCGGGGACTTCGCGTCATAGAGATTACAATATTCGATCGTCCGGGGCGAACGTGACTTCCGCTTTCCCATTCAGCCAACGAGTTTTTCATGTGAGATCGAGCAGCTACAGTCGCATCAACAGGCATGTGAAGGAGAAGAGTCCGTTTCAGCAATTATCAAGCAAAAGTCAGCGCCATCAGAGTGCGTTTCCAAAAATATAAATCAATAAAAAGCTGTGGAAGCAGCTAAAGCTTTATAACCTATAACCATATGTGTGAGTATCTCAGATATTCCGCACTCAATTGCAAACTGAACTAACTAACATTTACTTTCGCATCCTTTCAGCTTGGCAAAAGCAGTGGCCAGAGCGTTTTCGGGGCATGCGACTTAGTCACGGGTGTGCTGGTAGATGTGCCTCCGCTGCAGCCACATACTGTATGCTAGATTAGCCGTTAATGATTAGCCAAGGCAGTGTACGGCCAGTTAGTTAGTATTTATGCAATAGCAGGCATAGTCATGTAAGTGAAAAGCTACCGAAACTGCATTTTGAGCCGAAGCAAGAAGCATCAACATATACACACATTCGCATATGTTACAATTGTTTTATTAATCGGACCGAAAGGCCAGCGAGAATTGATTGTTTTGTAATTTGAAAATGAATAGAATTGTATACATCATGTTTGTAGTAGTTGCCAACAATTCGATTGTCTAATGTTGTAATTTCAATATGGCATTAACATTTGCATAAATACACACACACATAGTTCAAGCTTAATCAGACATAATGCATACGCATAACTTTTTCTATACAATACTTACCGATTTCAAACAGCGCCAAATGAACTTACACATAAATCGAATCGAGAATCGTTCGATATTTAATATAAATCAGCAACATTGATTAACATAGTTATATATCTTATAAGAAAAATATATATATTGCCGTCATTTAAACGCGCCTACAACTGTTTTCCTGCGGCGGGTAAACATCGAATTGGAAGCAAACAAGTTAAGCAAAATGTGGCGTGCAAA |
Search Again
