CRIMIC CR00624
CG14593
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
ATTGTCACCGTGCTGTACACGCTCAT |
GGCGAATTCACTCGAATATTCGAGCG |
Images
Construct Design Sequences
| sgRNA sequence |
GGGTTGGGGCGCATGTAGCATGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| AAAGTTCTGCCCCTACGCAA |
GCCCCAACCCGTTTTTAGAC |
GGCATGTGTAGGTGTCAGGA |
CGGAGGCTCCAGCAAGTTAA |
| Amplicon 5' |
Amplicon 3' |
| AAAGTTCTGCCCCTACGCAATGTGGTCTTACAAGCCGGATTCCAGATTCATCTTTGACCATTTACAAGCTGTATTCTGTCCCACTTTTATTGTTTTCTATTTTTGACAAGAAAACTACGAAACAGAGGCAACCCGACAGCTGGCGGTGGGGCTTTGGAAGGAGGCCGTCTGCTATGCAAATGTTTATTAACTTTTGAAACTTGTGCAGGCTTGTAACAGAAATTCAAATGTACATTGAATCAAAAGTTTCTGGGCCACGATGTTGGCTTGACAGCAGTCGCTAAGGAAGCCATTGCCAGAGTTTGGAAGCCACTGTCTCCCTCCCTTCGCTTTGGAGCAACTTAATCTGTAAGTGTAAGTGGTCATAAGTATACATTGATTGCCCGAAAACTGCCTCTACTACACTCCAAAATGGTCCCTGATTGCAGTGAGCCACTAGTTGGGGCAGTTATTGTGGTGGCCAGCGTAATGGTCCTGGTGGAAAGTTAGCAGTTTGCTCGACGGCTTTGTCGCGACTGTTTGTCTAGACACGATGACTGGGAACGTGTCAGGTTGGAATTCATGTCACATGCAATCAGCCAAATCCAGCAGTTCTAATGATTCCCCCGTCCGAGGACTCAACACTCGTTCTGGCTGGTTCACAGGAGGTCGTTGGTGTCAATGTCAAGCTGTTTTGCTCTCAATCGTTATTTTACTTTGCTCTGAACAGCATTCGTCTAAAAACGGGTTGGGGC |
GGCATGTGTAGGTGTCAGGACCTTTCCAAAGGATTTTGACTTGCCCTTGAAAAAAACAAAGGCATTCCAGCCCAAAGGAACCTAATTCCGGGTGAAATGGCAACGAAACAGACTTGATTAAATTCGAGTTATCTATAGTAACCCTTTGTCGATTTCATATATGTACTTCCAGATACATTCTTTCACTGGCCCTGGCTGATCTGTTGGTTATATTGGTGTGTGTACCTGTGGCCACGATTGTCTACACGCAGGAAAGCTGGCCCTTTGAGCGGAACATGTGCCGCATCAGCGAGTTCTTTAAGGACATATCCATCGGGGTGTCCGTGTTTACACTGACCGCCCTTTCCGGCGAGCGGTACTGCGCCATTGTAAATCCCCTACGCAAGCTTCAGGTGAGTCACTGTCGAACGACAGTTCGGAATGGGCTTACACATATTTTTCTTCCCCAATAGACCAAGCCGCTCACTGTCTTTACTGCGGTGATGATCTGGATCCTGGCCATCCTACTGGGCATGCCTTCGGTTCTTTTCTCCGACATCAAGTCCTACCCTGTGTTCACAGCCACCGGTAACATGACCATTGAAGTGTGCTCCCCATTTCGCGACCCGGAGTATGCAAAGTATGTTGATACCACTGCCTTTCCCGGCCCTCACTTGTACTACAATAGATCCATATGTCAAATGTTAGGGCCTGGCCATTAACTTGCTGGAGCCTCCG |
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