CRIMIC CR00463
CG8032
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
no band |
TGTTGAACAGCAGACTGTGTCCGATG |
CATTTGGTCAGTATGGACTGCTTGGC |
Images
Construct Design Sequences
| sgRNA sequence |
TGGAAAAATCTATAATCAACTGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GCTCGCTTTGAAATCGCGAT |
GGCTGGGGCGAAGAATGT |
GATTTTTCCACGGACTGCGC |
ATGTTGTACGGCCAGCTCAA |
| Amplicon 5' |
Amplicon 3' |
| GCTCGCTTTGAAATCGCGATTACGTTATTGCATTGCACATCGCATGCATTTATTTACAAAAATTATGTTTTTATCAGCGCGACGAGTAATTCGTGTACATAGGTCGTTGGAACTGTCTGGCAGCTCTGTTAGTGCTGGCAGGTGGTCGATAACAAGTGGAGTGTGATTAGAGCGATTACTTTTTATTTCAAATACTTTGTAATAAACTTGTTAGTGTCTTAATTAAAACAATTCAAATCTTCAATTTGAAATGTATATTAAAATCAAAAGTTTCATGAAATCACAGACGTTAAAGTATCGATACATTTAGTGCTGTAAAATGACAGCTGGCTTGTTTACCCTTATAAGCTTCAAGTCTGGCAATACTGCGACCAGCAGCTGTCGTAAAACTTATCCTTCAAACTTTCAATTAAAATACGTTTTTCTGAAAAAATCGCTTATCAAACTCCCATACATAAAAATAAAACTATTATTACACAATGTTTACTAAAAAAAATTGGATATTTACCACCCAGCCGAACAGCTGTTCGCTGCTACCTCCCCCAACCCATAAACCTACTGTCAAGTAAACGTCAAAACAGTCGCGCGACTCGCCAAGCGAAAAAACCTGCAGAAAAATTGAAATGCGGCGTGAAAGATACTAGAAAAATAACAAATAAACACCGGGTATCCGCAATTAAAACGCTTATGTACATAGAAGCCAAAGTGTAGCAATGGGCGACAAGGAGGAGCCCGTAACACCCGCTCCCTCGCCCACGGAGGAGGGTATGGCCAATATTGGGTCTGCTGGAGCCGGGGATCAGCCGCAAACTTCAGGGAATAATACCAACGTTAAGATCGTGATCATTGGAGCTGGAATGGCTGGCCTATCGGCTGCAAATCACCTTTTGCAAAACGGCTGTGACGATTTCCTTATTCTGGAGGCACGGGGCCGGGTGGGCGGACGCATTGTGTCCATACCGCTCAGCAATAATCAGAAGGTACCATTTGTTATATAACCCCCTCAAGTCCAAAAACCAAAACAAACCATCATAGGCGACCATATGGTATTAAAGGAAAAACACTTCCGACTCGGAAAAACCACGAGCATGTTGTAACACATTCTTCGCCCCAGCC |
GATTTTTCCACGGACTGCGCAGTCGGTCAGCTGTTCACGCTTTTCATTAAAGCTATTTTTATACATGTTCCTGTTCCCTCTATTTGCCTATTGTTAGCTTTATTTGTTACATATGCTTACATGGGGGTAATTAATGTAATTGCATCTGTCGTGAATCTTACTCACGATTTTCATTCCGTTCATTAGCATAGTTTTGATCGGTTTGGCGGCACCTTATCAAGAAATTTGTTATACAACTTTCTTCGGCAACGTTTGTCAGATTAGATAGGGGTTTTCTGAATTAAGCCAATGATGACGTATCTAGTGCAATGCCCTGAAAAACTCACGGGTTTGCGGGCTAACGATCACGTAGTCTTAGTTATGGGACTGAAAAATTTATTATTTTTCCAGTTTTCCACATTGTTTCACTTCACATGTACTAATTACGCCCCTAAATTATCTAATATAGGTCAGTTCTTATCTTTTTTATACTAGAAAGTTAAATGTTAAGCTTTGCCTACTTATCTGAGATGTTCCTATTTAAACACTTCTATTTCACAGATAGAACTTGGCGCCAACTGGATACACGGCGTTCTGGGCAATCCCATCTTTGAGCTGGCCGTACAACAT |
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