| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_PM37_p1 | 2L:23161122..23174484 | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | no band | GCTTGAAAGATGTCAGAGTGAGAG | GGTACAAAGAGCCTTAACTAGATTACAC |
| sgRNA sequence | ATCGGAACGCCCTCCAAAACTGG |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|