CRIMIC CR00491
CG15104
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
AGCGCCCGTTTTTACAGGTCTGTTCGTA |
TCCTCTTCCTCTTCTTCCTCCGCA |
Images
Construct Design Sequences
| sgRNA sequence |
TAACACCACACATTCTGAACAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GCTCACAAACGGTTGCCATT |
CAGAATGTGTGGTGTTATACATACA |
CAGGAAAACACTAGAATGATCTCT |
CCAAAGTTTGTGGGCTGTCG |
| Amplicon 5' |
Amplicon 3' |
| GCTCACAAACGGTTGCCATTAACCTGAGCATGAGGCGCCCAGCGGCGGTTGCCAGCGAGGAACCAGAGGTCATCGAGATCGACGACGGCGATGCGGCCGCAAATGCAGAGGTGGCGGCCATCAATGATGGCAGCAACACCAGTAGGAGACACGCAGGTGCTACTCTTCCAGTGACGGCGCACATCGAACTGGAGAGCAGCAGCAGTTCCGGCGATGAGGATGAGTGCGTCTTTGTGCTGGAGCTGAAGCCACCACACATGCGCACTCCCGAACAGGTGAGCCTTGACTCAAATAGTGACTCCGATGTGGTGTTCGTAAATGAGCAGCACGAAGCTGCGCCGGACGCAATTGCCGAAAACCGGTCGACACAATCCCCTTTGGATCTGGCCTCCAGGGATCAGGGCCTGTTTATGGGGCCCAGCACAAGTGGCGCAGCGGCCAACAGAGGCAAGAACTGGAAGCTGGTCATGGCGCAGACACGGCGCCTCGATCAGTTGCGCACGCTGCGCTCTATACGTTCGAAGAAATCACGCCGTTCATCCATGCCTGCCCGCAGTGACAGTGGCAGCAGCCCCAGCAGCTGCAGCAGCTCCTCCTTTCACTTCAGCAGCAGCAGCGATGAGGATAGCAGTGACAGTAGTACCACCAACTCCGAGCCACCCAAGAAAAAGTCACGCAAGCGAGTGGCGAATAATAAGCGCTCTAAAAAGGAGTCAATCGGGAAAAGGACATCTCGTAAACGGAAGGCCAAAGACCAGAATATGGAAATGCTTGAGCAGCAGCAGATAAGCCAAAAGAAGCCCCAGCGCCAACCGGAAAGCAGCAGTGACAGTCCAAGTTCGTCCGATGACGAGTCAGGTGGCGATAGCAGCGAGTCATCCGGTAAGTGGTTTGTGAATCTAGACGGTTCTTATCAGCATGTATGAATGTATGTATAACACCACACATTCTG |
CAGGAAAACACTAGAATGATCTCTAAAAACAATCAAAAAACTATTTATTATCCTATCAGATCAAACTTGTATAATTACACAAATTTGTTTTCTAGGTCAACCCAACACGAACAACAACAAATCCAGCAGCGATTCCGATGATGATAGCGCTGTAAACATGCAGCTCAGTGCCTTGCGCGCCACACTAAAAGCGGAGGCAACTCTGGAGGATAGGAAACCTGTAAAACTGGAGCTTCAGTTGCCGGATGACGACCAGGCGGGGCCCCTCCACTCACGGATGACTCCATCGCCGAGAGAGGATAACGAACCGGGTTGCAGTGCACCGAAACGGCGACGCAGTTGCAGCCACAGCAATCAGTCCAGCCAGAGTGCCAGTTTGGCCAGCAGTTCGACAGCCACCTCGAGCTCGGCTCCATTAAGTTCGTTTGCCTGGGGAGCAGCGGGCTTTTCCGGCGATCCGCTGATGCGCGGACATCCGGCGATGGAAGAGCACGACATAGCCAACTCCCTAATCGAACTGTCCACTCTAACGCAGCCTGTGAACATCGGACTCTTCAACGAGCATTATAATTCGGCAGAGAATTCCATGGGCATGCTGAGCAACACGTTGTGCGACAGCCCACAAACTTTGG |
Search Again
