CRIMIC CR00377
Gene Rx
CG10052
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
no band |
CAACTGTCAACAGTAAAACAAAGCGCCG |
AGTGTAGCGCGCTTTAGTTTTCGGG |
Images
Construct Design Sequences
| sgRNA sequence |
CCCGGCTTCACCCTCCATCGAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| TCCGATGTTGGAGGGAGTCT |
AAGCCGGGGGTCATGTGTAT |
TTGCAACCCCCGAACACAAA |
TACGTATCGCATGCAGTCCC |
| Amplicon 5' |
Amplicon 3' |
| TCCGATGTTGGAGGGAGTCTGCGGCCGGAGATGCCGAAGGATCTGGCATGGAGCACAATGTCGGCATCGTTTGTGTTTGTTTGCGATGGCATCCAATTTCATTCTTTTATGCGACTTATTGAGTTTGCTCTGCGCCGCTCCACCTCTCCTCTTCTCACCACTCCAATGTGTGTCCTTAAGGTCCTGGTCGGGCTCGGCTCGGCTCTGATGTCCCGATGTTCTGCTCTTCTGATGTCCTGATGTCCTTGCCCGGCCAAAAGGGTGTTGTTGTCTCCAGTTTGAGAGCCCGTGGCAGAGTTTAAAGGCAGCCTATGCCGCGTCCGCCTCCCAGGCGCATCCTTGCCAGCGCTTAAGCCACGAAACGTGTCCTTGTGCGCGCTATTTGCCTTTCCAGGACTTTGGCTATGATTGCGCTTTTCTGCGCACCACCCACCTGATGGCTAATACAAAGCGAATCGGCCGTGTAGTTGGAGTTCCGCCACGTGCGGGGGATTTGTTTTAGTTTTCGGCTCGGTTGTCTCGTATTCACCGTGGCCTGTCAACACATTTTAATATAAATGCATAAATCAAACGGAATGAAATTATAAATCATACACATGACCCCCGGCTT |
TTGCAACCCCCGAACACAAAACTCACTTGACATCACGGATTGCCTTGCCCGCCCTCAAGGTTGTCACTCCATTTGTTTGGCTTAGAGCGAAATAAAATATATAAACCAATGTAATCAAGCGAAGACAATTACAATTACAATGAAATTATTTCGCATTTTGTCGGTGCTTTTTTTTTTAATGGTAAATTGCTTTTGCCCAAAAATATCCTTGCATCTCTGAGAAAACAGCCAGCGGAGCAGCTGTTAATACGACCCTAAAATGCTCGAGTGTCAAGTGGAAAAGTGGAAAGTGGAAAAGGGGGAGTGGACGAAATCGGTGGGTGGGTGGTTTGTTTGGAGAGGCCATCACACCCCTCGGTGATGGGAACATTATGAATATTATTCAATTTGTACGCCTAAATGCTTTGTAATGAGTTGCACATCTGTTCGCTTCAGCGAGTGTTTAATACATCATTTTTTGCCTCTCGGAGTCAAAAGGCAACAGGATAAAGGATAAAATCCAAAGTGCGGGTGCTTTCGAGTATTAAGTTTTTATTGCATTACATCCCCGTCCTCTGTCCTCTGTCAGATGTCAAGGATGCACTCAATCCGGTTCACAGTTTCGAGCGCGATTCAATTATGACTCTGATTTCGGATTGTGTAATCTGGCTAGGGGATTGATGAACCTTGTATACCTTGTATACCCACTTCGCATCCAAAACATTTGGATGCCTCTCGGCTGTCAGTTTAAATGAGCATTGGGCTTAAGTTGTCAAATTAGCGGGCAACAATACAATTTCAATTTCGATCCACAGTCAATCAAAATAATGCCCAGGCCAGAAAATAAACGTATAGGGAATTTCCAAAGGTAAAAGTAAGGTGGAAAGGGGGACTGCATGCGATACGTA |
Search Again
