| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| pM37-p1 | 2R:24933845..24933867 | 60E5 | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | GTCGAACACCAGATGTGGTAAGAG | TCAATGACGACGGCATTTGTGTGC |
| sgRNA sequence | AGAAGCGGAGGCGCATTATTAGG |
| sgRNA2 sequence |
| Amplicon PCR 5' Left | Amplicon PCR 5' Right | Amplicon PCR 3' Left | Amplicon PCR 3' Right |
|---|---|---|---|
| TGTGGTTCATGTGCGGTGTA | GCTTCTGCCGCCTTAAGTCT | AGTTAGAGGGGAACTGGCGA | ATGCTTCTTGCGTCACTCCA |
| Amplicon 5' | Amplicon 3' |
|---|---|
| TGTGGTTCATGTGCGGTGTACGTATTAAGAAGCGCGGCAGCAGAAGAGTCAAGCGGGGAGCCCGCGCCGGATAGGAGGACATGAGATCAACCAGTGGCGATGTCATAAAACACACGGAAAGCGTAAACACTTGACGAATTAGCGGTAGGTGGCAAGAAGCGGCTTAAGACTAGCACCAAAGCAACCCCATACCCAACAAGTACATTCATTATCATCTGCTAATGTCTCTGAAGATCTGCTAAAATCGGCGAACTCTGCCGAACTTTGCTCGCTAGTTAGCAAACGAGGACGTAACCCGTCCATATTGGGTGTATCCATGGGCTATTTGCCCAGCTAAGCAGGTCACCCTTGCCTTGAACCCTCCCGGTTACAAATGAAATATTCATCCACTCCTATCGATTTCAGCATTCAGCTAGCCTATCATTAAATAAGTTTGAGGGGAGCTGCCATCGGAAATACCTATTCACAAACATTTTCCTAACCTTATCTGGGCAGTATAAAAATAAAAGATTCGATTAAAGTATACTTTCATATCAAATTACCACTCGGCTTAGTGTTTGATTTTGGAGATTTGCATCAATATGGTCGGACTGGTTTCTGCCCAATAGTTTCTCCACCTCCCATGAATAACGCCCTCTCAGGTTGGCCAAAAACTGCTAATAGAAAACTTTAATTGACTGCCAGCGCAGACAGATCGTACATTCCAGGCAGAGACGTCCAACAAAGACGCCAGGTGGCCGAAAAAGATATAAATAATAAGCAAGCCAGGGCAACATAGACACAGACACAGACATAGACATAGACTTAAGGCGGCAGAAGC | AGTTAGAGGGGAACTGGCGACTGGCAATTGCAGACTCAATTTCGCTTCCAGCGCAGCTGGCTACCAAGCAAGCAAGCAATCCCGTCCAGAGACATAGAGAATTAACGACTCTGATAAATCGATTCGCCGCAGGCCAAAGCGACGCGGATAGGCAACAGCGAGGTGGCATCCGTACTTAAAAGGTCACTTACCGATGCTGGGACGTGCGATGATGACGGGCATGGACTCGTAGTGGTCGCGCACCAGAAGCTCCGCCAGACGCTTTGAGTAGGTGTATGTGTTGGGGTGCGGCTTTAGCAAATCCGGCGTAATGGCGTCCAGGGTCTTCACGTCCATCCACTCGGCCAGGCGCATAAGATCCTCCGGCTTGTGTGGAAACTCGTACACCTTCTCATACATCACATCCTGGTCGCAGTTGCAAAAAGCCGTGGACAAGTGGATGAAGGCCTCCAGCTGCTTCATTTCCTTGGCCACGTTGAGCGCTCGCCGGGTGCCTAGCAAGTTCATATCGATGGCGTCCCGAAGGTTGCCCTCCAGCTTAAGGGTGGCCGCCATGTGGAAAACAATGTTAGTGTTCTCGGTCACGTGCTTCAAGCTCTCCCCGCTGAGCCCGAGCTCTACAAATGAAGGGCAGGAAATGCAAATCAATCGAGATTAAAGTGCGGAACTTAAGCGCTACACTTACGGTCGAAGGTGACGTCGCCCTGGTAGATGGTAACCCTCTTGAGCATCTCCGGGCGCTCGTCCTTGATGCGCTGGAAGATGGGCAGCTTGAACATCTCCTCGAGACGCGTCTCGGGTGCCTTGCCGCGCTTCGGGCGGCAGATGATGATCACCTCCTTGAGGGAGTGGCAGGAGTAGAGCAACTTCTCCAGCAGAACCTTGCCCATGAACCCAGACGCTCCAGTGATGAATATCGTCTTATCCTTATAGTATTCCTGCACTGGCGATAGATCCACCGTCATCTTTCTGCAAGAAAGAGGTGTATTAGCACACCTTCTGGGAATGGGAATGGAAATGGAGTGACGCAAGAAGCAT |