| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_LanB1_hcDNA | II:7811438..7820771 | 28C4-28D1 | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | L-F cctccaaactaacagtcgatacgaac L-R gcatagtcaggaacatcgtatggg  | R-R ACATTGCAGTGTTGGTTTTCGC R-F cgcatgattatctttaacgtacgtcac |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|