CRIMIC CR01709
CG7154
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| no band |
long band |
CGACCATGAAGTGTAAGCTGTTGTGC |
CCAACTTGGAGTAGACGTTTAGCTGC |
Images
Construct Design Sequences
| sgRNA sequence |
TAGGCATGCTCAAAAACAAGCGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| CGATCGACACACCATAGCGA |
CGGCAAAATGTGTGAGCGAG |
GTTTTTGAGCATGCCTACACT |
GGGCATTAAGGGAGTTGGCT |
| Amplicon 5' |
Amplicon 3' |
| CGATCGACACACCATAGCGATGGATATCCAGCGAATGCGTGCCGATCTGGACAGGATTCGCAACAAGCTGGACACCTCGCTGCAGAGCCTCAACTCGACGCGCACCCTATTCAGTGCCGCCAACAAGGCGAGTCTCAGGAAGTCGACCGCCCTGCAGAAGCCGACGCAAAACATGGTGACCTCCACCAATCGCCGCTCGGGTGGCAAACGAAAGGCCCGGACCGTTGCCATTCCGATGAACCGGCAAAGTGTGCTGACCAACAAGGCCCCTATTCTCAAACGAAAAGTTCGGTGAAATGCAAAGTAAAAAATGAGAAAAAAAAATGATTTAAACTTCGGCAAAAAAAAAATTATTTTCAAAATAAGGCATAAACTACAATAATTGTTCTTTTCAGTAAAGTATTCTTTCACTTTACAAGCATAAGTACATAGAAAAATTTACTGATAAAATCTGTTACTGTTTAAATCTTTGACATTATAAAATAAATTTAAATTAACAAATTTACAATTTAATTAACAATTTTTTAGTTTAAATTCAAAGCCGTAAAAATCGATATGTTATCAATTGCTTTTAGTGTTGGAAAGCAAATGGCTAATATCAGCGAGACGTCTTACAATTCTCTGGACAAAAGGAACGGTCACACTGCTCATGGCGTCTGTTTTTCGCCAGCGGATTTTTGTTTTTAATTATTTTGCTTACCATTTTCACCGATTTGCCTAATTGTTCGACAACTGGGAGCCTAAGATGGGCTCCTCCAAGAAGCACAAGAAAAACAAGTCGGAGCGACGCGAGAAATATGAAGGTGAGTGAAATGAAAGTGCTGTGTCTACGTGAGCCCCTGTTGCCATAGGCCATCTCGCTCACACATTTTGCCG |
GTTTTTGAGCATGCCTACACTTAGAAAAAAATGGGTGTACATTATTTGTCACAATTAATACAAAATGCATGGTTAGAAATAATCTACGCGTCTAAAGGCAGTAATAATACTATTCAGCACCCATTATAATACCTATACCTTATATAACCTGGCTTTCTCCTATATATATTATAGAATACAGTCAGCACCAGGACCCGGCTCAGCTGCAACGAGGTCTAAAGCTTATCCTTAAGGTGGGCTCGAATGCCACGCCGGAGTACAGTGCCAATTCGCCAATGGTGGACGGAGGACCGCCCACAGCAGCCGAGGCCATGATGTCGCCAGTCCCCGAGGAACTTCAAGATCACCAGGGTCATCGCGAGCGCCACAAAAAGTCAAAGAAAAAAAAGAAGAAGAAGGATCGCGAGAAGAAGCACAAACACCACAAGGAGAAACGGCACAGGAGCAGAGATAGACATCGGGACGCTGGATCGGACGAAGATATGATGGCCGGCGCTGACGATGCCGCTTGCAGTGGATTCGCCCCATCGTCTGTCGCTCCACCGGCCGCCGATCCCGATTCCAGCCAAGATGGATTTAGCTTTATGGACGATGATCAATCACAACCGCTGCCAGAGAATATTTTGTTCTTCGCCGGCATCACCACGGATAACTCGCCATCAAACTGCCCCGTAACAAAGCCAATAGCTCCACGCAAGCTGGATGATATTCTGATGGGCTCTTCGCCCAACTCGTCGTCGTTGCAATCTTCCTCCCTGGGTTTAATAGGCAGCAGTCCCACCAAGCCACTGCCCGACGTAAGTTACATTACCATATGGCCAAGTTCATTACTACATTCAATGCATTTTAGCTTTTAATACCTTCACCGTCGACGCCAGGAGGAGCCAACTCCCTTAATGCCC |
Search Again
