CRIMIC CR01146
CG31814
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
TTTAATGGCCAAATCCACGGCATCGG |
GAGGGGAAGGAAGGATAAGACCCTAA |
Images
Construct Design Sequences
| sgRNA sequence |
GAAGGTGGTACCATCTCGCGCGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| CCAAAAACCGTCTCCCTCGA |
ACCTTCATTGCGCAACAGTG |
CGTAAATGCACTGAAGGCCG |
GGACTTCCACGGCTAGACAC |
| Amplicon 5' |
Amplicon 3' |
| CCAAAAACCGTCTCCCTCGATGACAATCGAATGCTAATGCGGGCAATCAGCAATCGAAAGTTGATCTATGCCGAGGCACAGTATTAATGACCATTCCAATGACAAAAGGACAATGTCGGTCGTCGGCACGCCAAAGTCAAAAGCACCGCATTCCCACAGGACATTCATTCATTCAGTTGGGTAAACATTCAAACGAACCCGATGGCCAACACTTCAGCTCTGAGTGACGGAATGACAGGCTGATGGACAGACACAGACCCCGGGCGCATTAATAGAGTCAATAGAGAGACAATAGTTGAAAATTAACAAATGAATTGAAATTACTTAAACAAACTCATTAGACGCGGGCCGTGTCAGACCGGAGCACCCATCTCCGGGGACTTCCTGCGAACAGTATTAATATTCTGGCACAAATGTGTCGCCATCGCATGTAATTAAATTTTCCAACCTTAATGTGCTAAGCATTGTAATTTTCTCCCCCTCCCCCGACAGCCAGTCACGAGTTTAAATTGTCAGAAACTGTGACAGCCCGACACCTTTTCGCCCAGGTGCAGAGGAAACCGGCGAGGCGACAAGCCGCATATCTATAATTTTCATTAATTTTAATTGGCTTTCGGCGGGGGCGCAACGTCGCTCAATTACAATGTTTTCAAATTTTTATTGAATTTTAAACTTGTACAGGACTTTCTGCTCGCCAAAGGGTTCAACGAAGTATTTCAGGCAATTAAATGAGCATATTTAAATTCATGATTGAATGGTGAATAATAAGAAAGGTTTTATACATCGACTTTAGAAGGATAGACAGTAAATATTTTTAGAAAAAGGATGAATTCCTGTTGCCATTTAAAAACAAATTAGGTTTCTAACAAAGTAGGCTTGAGCTGACTGCCTTAACAGAATGACACAAACCTTTTAAAACCTTCTGTTTGCATACCTTTTGAATATTTTTCGAAAATTTGGGAAGCGTTGGGAAAACGGCGCGACCCCTCAAAATTCGAAATTTTTGTTGTGTTGCTGCCTTTAATTAAAGGTCCGATTAACTATGTCCAAGTTGGGTGCCATCCTGGCGCAAAGTGCTGTTCATCTCAGCGAGCATCGGCAGCTTTTGAGCCAGGCCTTCTTATCATCTATCACCTATCAACGGAGCCGGGTCAAGGTTCGCGGAAACCACTGTTGCGCAATGAAGGT |
CGTAAATGCACTGAAGGCCGAGGAGTTCGACGTTCTGATCATCGGAGGAGGAGCCGTGGGTTGTGGTTGTGCTGTAGACGCAGCGTGTCGTGGTCTTAAAACCGCCTTAATTGAGGCAGAGGATTTTGCAAGCGGCTCTAGTTCGCGAACCAGTAAGCTGATCGATGGCAGTGGTTCATATCTGGGCACTGCCCTCCGGGAGAAGGATGTCGAACAGCTGTACATTATGCTGCAAATGATGAGTGAACGGGTGACCATGCTGAAGAACGCTCCGCATCTGAACCGCATCCAGCCCATGATCATACCCATCTACAGTTTTTTGCAGATGCCCTGCACCTGGCTGGGATTGAAGGTAGGTTGACGAGCTAAGACTGGTTGCCACAAGTATGAAATATATATTCCAAGGTTTACGACTGGATATCAGCAGCATCCAATATCCGCGGATCGCACATCATCTCCAGGGAGGCCACCATGTATGAGTTTCCGCTGCTAAAAACCGATGGCCTTCGCGGTGGCGTCGTCTACTATGATGGTCAGGTGGATGATGCCCGGATGTGCGTCGCATTGGTGATGACCGCGGTGGCCCTGGGCGCCAATGTGGGCAACCACATGGAGGTGGTGGAGATTATGCCACAGGAGGGATGCTGCCGAGTTGTTAGCGTTAAGGACAAGATCTCCTGCGAGCAGTTCTACATTCAGTCCAAGGCCGTGATCAATGCCACAGGCTCCAGCACAGATGCCATCCGGCAGATGGACAAGGAGGGAACCGCACCCATTCTATTGCCCACTCTGGAAACCCAAGTATCCCTGCCCCGCTACTTTGGTTCCGGACATTACGGTTTGTTGAGTCCGGCTCTGAAAAATGATGATCTCACTGTATATATGGTGCCGTTTGAGAACCACATGGTTCTAGGCAGTCGCGAAGTGGACCTCGATGAAGTGTCTAGCCGTGGAAGTCC |
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