CRIMIC CR00866
CG10473
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
AGGATGTTGAAAAGGACGCTGTCGAG |
TACAAACGTCGGCCTAATTGGGAACG |
Images
Construct Design Sequences
| sgRNA sequence |
TCACTGACTCCCTGGAGATTAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GGTCCCCACTTCCACTGATG |
ACTGACTCCCTGGAGATTAGGT |
CAGGGAGTCAGTGAAATATGCCT |
TCAGGCGGACTTGGTCTAGA |
| Amplicon 5' |
Amplicon 3' |
| GGTCCCCACTTCCACTGATGATGACACCAAGCAGAGCGAGCCAGAACAAGAGGTAAAGGTTCCTTCTCCTTCCAGCAAGGATGAGGAACATCTAAAAACGACTAGTGATGTATTAGACATTCAGACAGAAGAGGTCGATGAAAGGGACGAAGAAGCCTCCGCTCCTGCGGAGCGCAGAGAAACCAAGAAGAGGCACAAGGAGCAGATAAAGGAACAGCAACCCGCAGAGGAGGAGGAACACAAGGAAAACCACAAGCAGATAGACGAAAACCCAGAACAAATTCACGATGAACCAGCAGATCTTTCGAACGACAAGCACGATCCATGTCTAAACAGCAGCGCAACCAACACAACCGGTGCTCCAGTTCTGCAGCAACAACGGTCGGTGCGGAAGCGAAAGTGGCTGACTAACAAAAAGTCTTCGGATCGTACCCCGCCCGTGCTTGCCATTTCGACGGATTCCCTGAAGAACATTATAGCCGATGTGGTGCAGCCAGTGCCCCTAAGCGATGTGCAACTGGAATCGTCCTCAGAGGAGGAAGGCCTGGTCACCAGCGATCGCGATTCAGAGCGATCTGCTTCACCGGCGCCCCCATCTGAAGCAAGCAAAGACCGCGATAACAAGGGCAATACCGCCAACACCTGCGACACCACCGCAGAGGCGTCGGCAGATAATGAAGGAGCAGGAGCCGCTCCTCCAGCAGCAGCAGACACGGTTTCAGCCCAGGTCAGCCTGAGCAAAGCCACAGTTTCCACGGCAGCACCACACATAGTCCGTGACCCTAGTCCTGCTCGAAACCGCGCCAGCCACGTGCTCTATATCACCAATTTGGTACGCCCCTTCACAGTGCTGCAGCTGAAAGGCCTGCTGGCGCGGACGGGAAAGATCGTTGAGGAAGATGGTTTCTGGATAGATCGGATCAAGTCTAAGTGCTATGTTGCCTATTCCACTGAAGAGTAAGTATTGTAGTTGCGCAAATAGAACCTAATCTCCAGGGAGTCAGT |
CAGGGAGTCAGTGAAATATGCCTATAAAAAACTCACACTTGCAATTATTTTGCTTTTCAACCTTTAAGCGAGGCCATCGAAACCCGACATGCCCTGCACGGAGTTCGCTGGCCAGTGTCAAATCCCAAATGTTTGAATGTAGATTTTGGCAGTCGCACTGATATGGATCGGGCAATACTTTCAACGAAGGACGAGGCTCCGAGGTACGGGCAGGAAAACACCAGAGATAACCAGCAGTCGGGCAACGCTTGGTCCCGGTTGGAACCGTCTGACAAAAAAGTAATGGATTATTATCACAGCATAGAAATGCGGAGCTAACTTGTCTTTTTCCTTCAGCCTGCGCGACCTGTACGCGAATGGGATGTTGGCAAAAAGGAACCAAGCGACCACGACAAACACAGCAACGACAGGCGTCGGGACAGCAAGGATCGACTGGACTCGAGGTCTAGGGATGCGGAGCGAGCCGGACAGGAACGAAAGCGTTCTAGGGACAGAGAGGGGCGAGGACGCGATCGGGAGCGGGAGCGACTTGATCGCAATGCACATGCAAGGAGCCGCAGTGGATCGCCAGGTAAAGCCCGTGGGATACATTTTCCTGTCATGAGAAATTCAAAAACACCCCGTTTAAATAGCGTCCAAAACTAAGAAGGAGAATGAACCGCCTATCAGACTACTAGATGATCTATTTCGAAAAACCAAGGGAACACCTTGCATATACTGGCTGCCACTTACGCCGGAAGCGGTAAGTTGCTGAGATAAATAGTTCCAATCAACGAGAATCACTTCTAGACCAAGTCCGCCTGA |
Search Again
