CRIMIC CR00322
CG31132
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| short band |
OK |
GCTCCGATATTCATAACTAGTGGTGTGCG |
ATTGCCAGAAGGCGATCGATCCATCTGT |
Images
Construct Design Sequences
| sgRNA sequence |
AGACAAGCGGACGTACAAGTGGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GGCTCCTTCGGCTAAGACTC |
GCGGACGTACAAGTGGGTT |
TGGGAATCAGACTGAATTGTGGA |
CCGTCCAGTTAGAACGCGAT |
| Amplicon 5' |
Amplicon 3' |
| GGCTCCTTCGGCTAAGACTCGATACCCATCTTGTGAGCCCCGCCACCAACTATCGATACGCAAAGGCATTTCATTCGGTGGGAATGGCAATCGAGTGTCACACGGGAAGAAGAAGCTGCGAAAAGAATTTTAGAAGAAAGCAACTGCAATTTTGTCATAATCGGAGCTGCAATTCGCAATGAATAATGGAAACTAGACAACCCAGTTCCGGTGATGATCTAAAGCCAGGTGCGTACCGCCCGGTGGCGCAGCGAGTGCGTCCAACGGGGCTACCAAAGGCAGCCCCTTTGACTTTGTGAAATGTGTGTCTGTGTTTCGCTCGCCCTTCGAATCGAATGGCTGCGTTTTTTCGACTGCGTTTCGCCAAGCGAAATTCACGGGCCCCTTTGGCCAGATATATCTGGAATATTGACGACTCCCAGCGAGCGCATTTTCGACAACTTGTTGCACTAAAACCCGTGAAATCCTCTATCTTGCAGAGCTGTACTTCCTCATTTCCAAGTTCCTGGCCGCGGGACCGCTGGAGGAGACGGCGAAGGTGGGTTGAAAGCAGCGCTCGCGCGAGATCGCATTGGAATCGTATTTCGCGCTAAAAGAGCGGAATTTCGTTTTGTTTACAAACATTTACATTAATTCTGAAACTAAATTCTATCCAACTGTAGGTCTTGATCCGGGAACTCGAAGAGAAGAAGGTGAGTTTTGTTGGTGCTGCAGCTGTGTTTTTGGCACAGATATTTCGGACTCGAATTGAGAGGGGGGCCAAAAAAAAAATAGAAAACCGCGTTTGTTTATACGCTTTGTTTTGGTTTTGTTGTTGTTTCTGCTGCCGGCAGCGAACGACGAAAAACCCACTTGTACGTCCGC |
TGGGAATCAGACTGAATTGTGGATTTGCTGGGGCCACGTGAATATATAGTCCATTAAAAAAAGAAGAGAAAAACATGAAAAAAGAAATGGAATCATAGGGATGCCCCCGCGACGTCATCGTCGACGTCACGGAGTGCAAGTGATAAGAGTCATACATGCATGCATATATATTTACACCCGTCTATATATACGTACACCTTTCGATTCCCAGGTTTTGCCGCGTCGTCTCGATTGGCAGGGAAACGAGCATGAGCAGACCTTTGAGGAATTGGTGAGTGTTTTCATAATTAATCAATTAGCTTTTCAAAGTTATCGTTTAATGCCTTTTGATCGGCATTTAAGTCTTGCGTAAGTGTGCGTCTGCTGCGGCATTTTCCGTCTCAGTCGTTCGCTCGCCTTTGTGACGTCATCGTGTCAGGGAATAGAGAAAAAGTTGCACATCAAAATTCAAGTTGTTAATTTAAAAAACATTACAACTAAAGACATCAATCATTCTGACTGTTAAATTAACAAATAAGTAAGCAACTATTTAATTAAAAAGGCAGCAAAAAATTGCAAAAATTCGTAAAAGACTAAAGTGGAAAGCAAAAATTCTAAAACCCTCGTAAAAATTGTAAATTTTGAAAAATCTTAATTTTTTAAACAAATGCTTTGTAATTTAGTTTTAACAATTAGCTGCAACCAGCCGACATTTTGGTTTCTCACACATACACATGCCCATGCGCACAGACACATGCAAGCAACCACCACTTTGCCTTGACTTGTGCGCGAGGGAACGAGACAAGCATACGTTGTTGAAGCTATCGCACCGTTTATCGCCCAATCGATAGGAATACGTTAAATTTTGATTTTGAATTCGCAGGAACGAAAGTACAAGCACATTGGCGCCAATCACTTGCTTGAAATATGCAGTCGTCTGGGTCCACTGGTGGATCGCGAGCTTCCGCCCAGTGTTCCGGGCATCAACTCGCTGTTGGGTACTGGGCGCCAGAACCTCCTGCGCACCAAGGAAACCGTCTACTGCCATCGCTCCTTAAGGGACTACTGCACCCGCCTGAATGGAGTCTCGCTGCCGGATTCGGTCCTTACAAAGCCGACCCACAACTTAGGTTAGTTGCATGGTAGAGTTTTATTCGCATATTAGAGACTAACCCATTTATATTTCCAAAGATCGCGTTCTAACTGGACGG |
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