CRIMIC CR01646
CG31612
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
GTCTGCCAGTGATGCTTGGTGCTTAT |
CGCTCTTTCCCACATAGCTGCAGTTA |
Images
Construct Design Sequences
| sgRNA sequence |
CCAGCAAAACGGACTCGGGGAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| AGACGAGTTTCATGCCACGT |
TTGCTGGTCCCATTATCCCT |
GCCCCAACATAATGCTGAACG |
ACCCCATGGCTTGACTAACG |
| Amplicon 5' |
Amplicon 3' |
| AGACGAGTTTCATGCCACGTCCACGATTGTCCAGCCGCAGCCAAGTCAAAAGGATCCGCAAAACAAAAATGCAAACTTTCGTAGGCAAAAGTTTCTGCACATCAATTACGTCTAATTAATCGAAACAGCTGAGGGTAGTCCTGGTTCCTAGTTAGCGCTTCGAAATGGAGCAACATCAGGATTCAAATAAGCCGGCACAGAATTTTTGGGTGATTAAAGTGGCTGTAGTGGTTCCATTTTTAAAAAGCTGTGCTTTAAAGTGCTTTAAAATCGCCACTTTTAGCTATTACTAGTATAAAAATATTAAAATGTATTTTTTCAACTGTGTTGCCTCCTCTCGGCATGCATCGTAGTAATTTGCTTAGATCATTTCGTATTCAAGGAGGAAACAGATTTCTCTTAATAACGCGCGACATTAACAAAGTTTCAATCTTTAATCTAAGTTCTCGTTTCGAGACAAACCCGAGCAACGGATTTGGGTGTATGCGGCATTCTCTTGAAGGGGGAGAGACAACATTGTCATGCATTTGTAATGATGCCATCGCGAGTTTGAAAGGAAGGGAAAGACAGGGGCGTCGTTGATATGTGAAATGTCACTTTGTATGGAGTTTGAATAGCAACGACAATGACAACGACTCTGGCACAACAGCATGGCAAGGAGGCAGAGCTGTGGAGCTCGGTTGTGGAGTTGGAGGGGATGTCAGATGGTGGGAGGCCGCTACTCCTTGAAGGTTGTGCCAGACTTGACAAAAGGATGCCATTTCACCTGCACGGTTAGCCGTAAATTGGTCTTGTTTCTGTTGCTACCGCTGCAGGGATAATGGGACCAGCAA |
GCCCCAACATAATGCTGAACGAACAAAATTATCTTTTGCTGGGTTCGTTTTCCCAGCTTCCATTTGTATTTTCACCTCCGACATTAACTTTTATTTCCTCAAATGCATTTTCTTCTCGGGAAGTTCTTGAGTTTCCCTAGCCATAACCGTCTGTCTCGTTAATACGTGTGTCCGCCCACAGAAAAACAGAAGAAATGTTCTCGGGTCCAGACCGCTCAACATTACGGTTTCAACTGGATTGCAGCATTTGGATTAGCTCATTGTTCTCGAAACAATTCAATTATAGCTTACTCCACATATCAGGCTACCATCGGAGCGAAAAACATATAAATCGTTAGATACTCGTATATTACATATGTAATCTAAGTTTCTAAATATTTCCCCCGTCCATCCAATGAAACCTTAACTCAAGCAGTCCCTTAAAATAGTTTACACATTAAATGCCTGCAGTTTGGCGTGCACATATAATTCGCATATATTCCCAAGCCAGCTTCGTTGCCAGGCTTAATTTCCAAAAGCTGTTCAAAGGCGAATCTCCCTGAGATAGGACGCCATATCCTGCACCTTCGTTGCCGATTAGTTGACGAGAACGTCTTGCGATGAAAACTTTGCCCTTTTCAACGCTCCTTTCCAGGCGTGGCACGGGCTAGGGTGGAGCTGTCATCAACACCCCCGCCATGTGCCGAGGAGGAGCGCAGCATCTGCAAGCTAGGACAAAGCGTTAGCTCTGTCCGGGTGTTGTGTCAACTTTTGCACGCCATCCATTAGAGAGATGCTCATGCCCTCAGCGGATGCTCCTCTCGTCGCCAACGAAATCGCTGAGCCAACATGTCACGGGTGCGGCAGGTGCGAAATGTATCCTGCTCCTCATTGTCGGCTGCACGAAGATGCAGGATGTTCGGGGCTGGCAGGTGGAGTGCGGTGGAGCCACAGCTCCAAGAAGGACTCCTGTCTTTGGCTGTCTTAGACAATCGCATGTGTAGTTTGGCGCAGCTGAATGTTTGCCCAGGCGCGCTCGCATTGTTGTTCCCCAAACTCGCATAAATATTTATGACAGCGTCTTTCTTGTCTTGTCTTGTCTTTTGGGCGAAAACTTCGGGGCCGCGGGGCGTTAGTCAAGCCATGGGGT |
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