CRIMIC CR01719
CG4609
Information
| Donor |
Location |
Cytology |
FBti |
Stock number |
| pM37-p0 |
3L:.. |
|
|
Order from Bellen Lab
|
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| no band |
OK |
CCATCATCATCAAGGAACTGTCGTCC |
CGCTCTCATCGCTTTCATTCATCGTC |
Images
Construct Design Sequences
| sgRNA sequence |
GGATGCACAAGCATACGTTACGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GCGCCTAGCTATATAGGGCC |
CCCGTAACCCCCATCCGTA |
GGTGGTGGAGGTGATGTGAG |
ATCACCTTTGGTCGCAAGGT |
| Amplicon 5' |
Amplicon 3' |
| GCGCCTAGCTATATAGGGCCTTACTATATAGTATCGTACTACATATGTATATATATATAATGTGTGTGTATGTGGGTGTTTCTGTTTCCTTCGATCATACACATATACTAGATATATGTACGCATATAACTAACGGCGAATTTACTTTGTTTCCTCCTTCTCTTTGTTCTCCTCGGTCGACTCCTTCTCGTTTGACTGCAAAATAGAAGGACATGGCTTAGTATGTGGCTAAAGGTAACTTAAATTCAAGACTAGAGGGTCTATCCTTGTCCTTGGTGTGTGGAATTAATTAACAATTTACAAAGAAAACCCAAGAATAGACCATCTGGTTTACAGGACTCAAGACTCACCTTGTCCTTCTCCAACTCCTTCTCGATCTTGTCGCCCTCAGTGCCCTCCTGTTCGTTTGATTTCTCCTGCTCGCCACCCTCCTTGCCCTCCTTGGTCTCGGGCCTGTCGGAAAAGCGGGAAAAATGAATTAGTACACCGATCATCCATTGGAAAGTTTTCATCCAAGATTACTCACTCTGGCTTGGGAATGTGCGCATTGAGGTCCAGCTTCGTGCAGATCTCGTCCCAGTCGGGGAAGCACTTGTCCTTCATGCGAGATACGTGGCCAATCAAGTTGGGGCACTTCTCGGTCATGTAGTCGCGCAGCGGATACGCAATGTCCTTGGACAGATAGTGGAGCTGCGAGAGGACAGCGAAGGCCACCACATCCAGGGTGGTGGGCTCGTCGCCGAAGAAGAAAGGCTTGCAGTCGAGCATCTCGCTGAGCACCTTCAGGTCGTCCTTGCCGAACTCCTCGATCTCCTCGGCGCTGTGGACACCGATGCCGTGCGCCTTCAGCTTCTTCGTGCCCTGGAACCACTTGTTGTGTTCAATAGTTGTTTTGCCGTACAGCAGTTACGGATGGGGGTTACGGG |
GGTGGTGGAGGTGATGTGAGTTTTTAGAGTTTTTTTTTTTTTGGGATCGGGGGCATTACGCATTACACAGTTAGATGTTCAAAACCAAAGTAGTATGCAAAAAAGAACGAAAAAGAAAAAAGAGAAAAACCATGCAAGAGTTAGTCGTAAAATAATGATTGTTTTCCTTTCGATTTCCCGTTTGGGTTTTGATTTTGATTTGGGTTGGTTATGTGAGAACCGATCGATTTGATATAAACAAATGCATCCCGCCAGAGAATTATTGTTTATGCCATCGGGGTTTTCGGGATAAACTATGTGCGGGGATGGTGGTGGTGTGTTTGGAGTTTCTTTTCTTTTTATGTACAGCTAGGTGCAATATAATACGCCACCGCTTGCTTAGTTTTAATTCTCAAGTATTGTTTATTTACACTACCCATCCATCCCCGCCTAATCCACAACGCCAGCCAACTCGAGAACCTACCCGGCTTTCGATTACGTTTTCAATCTAAACAAAGTGCGGATATCTCCGGTTTTTGGAACTACTGGAGGCTCAGCTGCTGGCGTTGAGTTTCTGAAACATCTAAATCCGCTACACTAAAAACATTTCTCTACTTGACTAGCTTCCGGTTTCCACTTTCACTTAGTTTGTCTGATTTCGATTAACACTGGAGTTCAATAGATTTCTTTTGGTTAAAGATAGAGTACAGTCTCTTTCAACAAAATGGTAATAATATTCACTTAAAGCTTCAACTCTTAAGATCACGAAAGAATATAATTGTTTAATATGCATTTATGAAATGTACAAATAAAAAATTGTACCAAAGTATGAGCTATTTGTAATTAAATGTTACTTTTACGAACAATAAAACCTATAAATTTGTTTTATTGTTCAACTAGAAGTATGTGTCAAAAGATGTTGATATATTAAGGAAATATTAGGTATGAATGATAGTAAACAACTTCTGTAGAACCAATTACGATAATGACCAAAAGTATGCAATGTTCCAATGAAAATTCTAATCATAACCATACAAATTCGGGGAAAGTGAAGATCGTGGACCACGATCATGACCTATGTATGTTAAATATCACAAACTATCCAGTTACCTTGCGACCAAAGGTGAT |
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