CRIMIC CR00802
CG9397
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
AAATCCCAAATGAAGCCGTCTCCTGG |
ATGAGAGATCAGCTGCCGAACTAACC |
Images
Construct Design Sequences
| sgRNA sequence |
GCGTTTCCTTACAGATCGGACGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| CAGCAGTGGATCTTCAGGCA |
AACGCAAGCTTATTGACCGA |
GGACGGCTTTGATTCTCGGA |
GACAGGGTGCAGGAGTTTGA |
| Amplicon 5' |
Amplicon 3' |
| CAGCAGTGGATCTTCAGGCAGCTCCTCGTCGTCCTCGTCTAGCTCCTCAAATGGCTCGAATACGGGAAAATCGCCAACGAAGAAACACGTGGCCATCGCACCCAGGACGCCGGAGATGAAACAGCAGCAGCAGCAGGGAAAGGCGGCCATGGTCTACCGACCGCCAGTAACAAGCACCGCTTTGAAGCAAAAGCAGATCTCACCACCCGCCCCCGTTGCGGCCCACACACCCGCCCCAGCGCCAGCATTAAATCCAACGCCCGCCCCCGCCAATCCCACGCTTTACCAGCTGCCCGTACAGCTTCCTAATCTAGTCCAGCTTCCGCCTCAGCTGGCGGCTGCCGCCAATATTATGCAGCTTAATAACGTGGCCAAAGCTGCAGTGGCTGCGGCGGCCACCAACAACGCGGCCGCGCAAGCGGCACAGGCTGCTCAGGCAGCTGCTGCCCAGTACTTCCTGAACGGCACTGTCTTTAAGCTGCAACAGGTGACTACGGCCACGACGACGACGGCCACCACGGCCACAGCAGCGGCGGCCCCAGCTGGCAATCCCTTTGGACTGCTTAACTTAGCCACGGCGGGCAATCCCTTTGGCTTACCCAATGCCAATGGGCAATTCCCCATTGAAGCCATTCCGGGAGCTGGAAGCTATCTCCATCATCAGCTGCTTTTGGCCAGACAGAACAACATGAGTTTAAACGGTAAGTATAGTATATTTTTAGTTTAATTAAATGAACTCCGAAAGTAGGAATAGCTAAGCTTCTAGTTCGACATAACTTGCAGAGGCATAAAACACTAAAGAACTCGAAAAAGTCGGTCAATAAGCTTGCGTT |
GGACGGCTTTGATTCTCGGAGTTTTCGCTTATGCTTACTTTTCCATTACAGAAACTATGGCTTCCAATTCTTGCGGCAACATGAACTTTGTTAATCCACTTAACAATCAGCAAGTTGGACTCAAAGATAACAAATTATATATTGTAAACTCGGCCGAGTACCTAGGATACTTAATGAGTCTGCAGATAGCTCTTAATAACCAACAGCACCAGCAACAACAAATCATATCTGCCACACAGCCGCCACCACTGGCAGCCACAAACAATAACAGTACGAACACTGCCACTCAGCCGCCGCCATTGGCAGCTACGAACAATACCAATTCGAACACTGCCAACAATTCGACTGCCAGCAACAGTATCAACCATAATGCCTCGAATAATCTAAGCAATATCAACAACACTGCCATACAGCCTCAGCGGGCGATTGTGAAGCCGCCATTGCACAGTTCCACGCAACCGCCGCCGCTTGTGACGATTTCCTCCATGCCCGCGTGCTCACCGGCTGCCGCATCTTCGCCAGCGGCCAAACGGTCACTGCCCGCCGCCAAGCCTTACCAGAAGCGGCTGACAGCAAAGGGCAGAGTGGGGACAGCACCGATAATCGCAACGCCTACAACGCCGCCTCCTCTGGTACCCACGTCTGCTACTAAGGAGCTGGGTCAGTTGCGCAAGAGTACGGGAACCACGGGAACCCCTACTGGCACACCCACACCAACGCCACCGCTAGTAAGCATCGCACCCTCGAAGCTGACCCCAACGCTGAGCGTTTCGAAGCAAGGACCGACAATGAAGCTTGCAAACAGCGCTCCCGATCTCTTTGACCTGGTGAAGAACTCAAAGCTGGTAGCCAAGGTTTCGCAACCGTTGACGCCGCTTCCCTTCAGTTCCCCCAGCAGCAGTAGCAACGGAAGCCACGGTAGCCACGGTATGCGATCCTCGCCGGCCCTCTCCACGTCAAACTCCTGCACCCTGTC |
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