CRIMIC CR70541
CG5553
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK? |
OK |
GAAATATCTTAACAAAGGCAATAGCGTCG |
AGAGAAGAACTTTATGGGCAAGAAG |
Images
Construct Design Sequences
| sgRNA sequence |
TTTGGCGCGCAAACTACTGATGG |
| sgRNA2 sequence |
AGAAAAGTTATATTATTCCATGG |
| left_200bp |
right_200bp |
| TACTACTGGACACTATGTAAACAAAGCTAAGGCCTAAGAAATATCTAACAATGCAACACACAATCAATACATCAGTACCAATTGACTGATAATAACAATTAAAAACGTTTAATTTTGAAATACAAATTGAATAAACCGCAAGCATCGATAATGAGCAGAAAACTATCGATAACAACTGACACAGCTGATATTTGCCATCA |
CCATGGTTCGGACTTCACCTCCGGCACAAaTAGCACGTTTTAAGCCTAGGATCAACAGTGTTCTCCGAGTTTCGGCCGTAAGGaGGCCAGCCATTGACATCTACGTAGTAGATGCCCCTGGCTTGGGGATTCATTCGATAGCCCATCGGGGAAACTATGCCAGCAGAACAATCCCGTTTTCTTAGCTCATCCCAAGAAGC |
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