CRIMIC CR01488
CG7904
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
short band |
TTCCTCTGTAATGCAAGCCGACTTCG |
TTCGAAGTCAAAGGCAGCAGCAACAC |
Images
Construct Design Sequences
| sgRNA sequence |
TATCGGTTGGAAGTGCTCAAAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| AAAACGAACCAGCGAACGTG |
GCACTTCCAACCGATAATTCCA |
GTGGGCAAATAAAGTCGAGCC |
CGCCAAACGCATACTCGGTA |
| Amplicon 5' |
Amplicon 3' |
| AAAACGAACCAGCGAACGTGCACAGGAGGGCAGAAATTGCAACACATGCGGCTCCGAAAAGGGGTTGCTGGAGGCTGGGGATGTAGAAAATGATGGGGGAGCGGGCGCCACTTCGCGTCAAGAGTTCGTTAACACGAAAAACGCTGCCTCGGATGCGAGGAGTGGTATTTTGTAGGCGAAGCCTACGCCCTCTTTTTGCAACTTGAAGATGCAGTAAAAATACAAAAAAAAAAAAAAAAAACGAAATAACAAAAACAAACAAAAAATATATGCAAACGGAAAAATAACAAAATTGGGGTAAACACTCCAAAGACAAAACCCCAGTAGCAGCAAACGTTAAACCGAATCTGAATCCGAAACCGGATCCGAACCCACCTTGTGTGGTTGCCTCCGTGGTGCTTTTAATATATTTCTGGTTGGAATTGCACCGCGATCCCTTGCAGCAGCAGAAGTGAATGTTTCCCTGCCGTGGCTCTGCACTGGTCACGCACTCCGTCTGATTGCATTCGTGCATGTCCGTGAAGCAGCCCTTCATCTTGATGCGCAGGATGCCTATGGGAACGGAAAAATTAAAATTAATCAGTATGCCTGAAACAACGTCAACTTCTGCACGGCTCACCCGTTGTCTCGTTGACCGACCAAAGGACATAGCAGCTGGGAAACTTATCCGCCTCCATCTTACAGTGCTCTATCCGTGTTTCACATTGCTGCGTTGTGTTGCACATCTTCTCGTCGAAGTGCTCGCATTCTATGATCCCATGACTTCCGGGCAAAATAGATCCATGGATTCCAATCAGACAGCAGACTAAATGAAGACAATGGGAAAATAGATTAATAATCTTTCTAGTTTTAGATTAGCACTGATCTATATTTAAAGTATATATTTGAGCAGTCCTAAAACATGAGTAAAGACTTTCAAAAAGTTAAAACGTAGTTAAAGATCAAAGTTAATGGAATTATCGGTTGGAAGTGC |
GTGGGCAAATAAAGTCGAGCCTATTTGGGGAATAAGACTGAAAGGGAGGGCGCTCAGCGAGCTCTTTGTGGGCGCTATCCTTTGTACACATATCGCATGCCCAGAAGTAGCAGGAGTAGCGTCCTAAGCTGGGGCCTCTGCCATTGAAAGCCACCCCATTTGGGCACGGAATTAGCCCCACTGGCCACACACCAGCCTTTGAGTGGGCATGGCCCCAATGCACGGATATGCAGTGCACAGTGGGCCTGCAGTAAGGTTTCTTGAAAATCTATTTACATTCTTTAATTCGATTTAATCTGGGATCCTCATCCAAATACCGAAATTTTTGTTGATAAAAATTAAAATGATAATTCACTTTTTAAATGTGTAAATACAAAAAATTTAATTTTGAAAACGTTTATTGCAACGAGCCTGCGATATATTGATTGATATTTCTTGACCATTGGCTAAGCAAACTGTTTTCAATCCTTTGAAACACTTTTCGTCCCACTGTCCTTTAGGTAATCGGATCGCTGGCCATGTATGTGTGTGTTCGTAAGCGTGCATGCTTTATAGTAAAGTAGATACATAAGCAGGCACATTATGCACACAGAAAGGGATGGGTTTAAAGTGGCGCTTGTGCGGAAAAAGGAAACAGCAGCAGCAGAAACAACAACAACAACAGCAGCAACAGGGAAGAGAGCGCACTGCGCCACAGAAAGAGAGAGGGCTATGGCGAGCGAAAGAGAGGGAGGCCGGTGGAGCAAAAGGGTGGGAGAAACCAGAACCTGCCATACAACAAGTTCCTAATGCAGCAAAATCCATTCAAAGAAATGCTCAGCGCACACACACATGGATTAAGATTGGGAATTAGGTATCCGATCGATTTATACCGAGTATGCGTTTGGCG |
Search Again
