CRIMIC CR00829
CG34174
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
GGCGACACGCCCAGTTTATGTATGTA |
AAGGAATTGGACAGTGCTTTCCTGGG |
Images
Construct Design Sequences
| sgRNA sequence |
AGGGGCTGCTTTCGACAAAGAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| ATTCAAGTGGCGTCCAGGTT |
TTCCTACCGGCAACTTCCAT |
TGTCGAAAGCAGCCCCTTTA |
GTGGGCGACAAACGATATGC |
| Amplicon 5' |
Amplicon 3' |
| ATTCAAGTGGCGTCCAGGTTAACATATTTTTGAATTTGGCTTTAGCAGTGATCTGATTGAAAATTCACAGGAAAATGAAGGATTTCTACACACAATTACCGGCACTTTTGACGAGCTTTACTTTTTTCTGAATGTGGTGTGTGAACATTGATTTGGTCGAAACATATCGATTATAAGCTTTTCAATTGTGTGTATTAAAGCAATTGATTCATGTAATCGATTAAGATTAAATGAAATAAATGTCGAAATGTCCATTCCCCATTTAAATAGCTATAGTTCTATGCTAACATTGAATATATTCACTTATCGATATTAGAAACCATTAGTGTTGATAAACGCGTTGAACGTATTTCCCAAAGAGGAGGTGTGCATACACTAGCTGAAGTAAACAATATTGTTTACAAAATGACCAAAGCGAATAAAACGCCGCAAAAAGCAAGTGAACCGAATATAAATGGTTCCTTCACACCAGTTCGATGTAAGTTGGCTCCAAAGACAAAAAAGGTTATTATTTCTTTGCATCGTTTCCAGATTTGGACAGCCAGCGCCTGCAATCGCCCACGAGTCACGATGCGAATTTAGCCACCTGCAAAACCAGATTGGAAACAATAGTCAAACAACTCCAGGATAACTACGCTAAATGGCAGCTGGCCCATCAGCGGGGCACCTCTATTTGCTATACTATCGAAGCCAAGAAGACCAAGTGTCTGGAGAAAAGCCAGGATGAAGGCAGCTCCCTTTACCCAGATGATCTCCTACTTCCCTGCAACAAGTTGGCCATCATAGCCTCTATTTTCGGGGATATCGCAAATAATACAAAGGAGATACTAAGACAACTGAGAGGCATATTAAAATTACCGGGATCTGCAGCGGATACGATATTCTACAGGTCCTGGAAGTTGCAGCAATTCGTGGTTTTCGCGAAGGAGCTATCCGAAAGATATGAGAAGGAGGCTCTGGTCAAAATGGAAGTTGCCGGTAGGAA |
TGTCGAAAGCAGCCCCTTTAATACGTTTGAAAATTCAAAGAAGCACAGATTAATTTCTCAAATTCGATTTACCATCTTAAATATAATCATGTTACTTTTCTTATAGGTAACATTGCTCACTCGACTGAAAGAAGCCAGCTCATTGCGCACACAACTTTATGGGAATTTCCCGAGCACGTGGACTCCTATGTCCATCTGGGGTTTCTTCTCCTTGCCGAAGAAGTCAGTTTAAGGCAATAAATTAAACAATTTTTCAACTTAATACAACATATGGTTTCTATCAAATGTTCATGTTGGGCAGTAAAACTTCATTACAGCTTTAGATTAAGCGAATGCTTAAAGAAGTAATAAGTAAATTCTATAAACTTAGGTCCTATGAGAAAATCGGAATGTAACTATTAAATGCATCGTTGTGCGTTTTTTTTAAGTCTGAACGGAGATCCGCTCCAGTCGGGGAGCGCTGCGGAACTTGGTCCACCACCAGAACCAGGCGCACGAAAATCCCACAGCCAAGGTGGTGTAATACAAAATAGTAGCCAGGCTCCACTTCTTCTGCAATGCAAATTTGTATAAAGGAAAATAATTTCTTATTGCTTGCTATATTCACCTTCTTGTGACTCTCGTTGCCCTGCACTTTCTTCTGGCTCCTTGCGTTCTGGGCATTAGCTTCGATTTTCGCGAGATCCTTGGCAATCTCTTCGGTGATCTGATTCACGCTTTTTAGAGTTTCCTGGGCAAAGCAGCTGGCATATCGTTTGTCGCCCAC |
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