CRIMIC CR01144
CG5423
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
TTTCATTGTCCTTTCCCGGCGAATCG |
TCACCTGAATGCGTTGCGTCGTGATA |
Images
Construct Design Sequences
| sgRNA sequence |
GAGGGGTTGACTGGTGTAAAAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GGCACAACTTTCGCTTCGTT |
GACAGGCACAAGTATCCCCG |
TCTCTTGCACCTTGCTGTCC |
TGCGTTAAACTGTTGGCGTG |
| Amplicon 5' |
Amplicon 3' |
| GGCACAACTTTCGCTTCGTTTCTGGGTTTTCGGGTAATAGGCATCCGGTCATAAAAACATGAATTGTGGCTCTTAGCAGCAAATCCAATTGGGCATAAATCGGCTTTGTCGGCAGCACATGAGATGGATCCTTCCTCTCAGATAGCTGCTTTTACCTCCCCGGCTTCCTTTTAGGTTCTTAGGTCCTGGCTCGTTTCATGGCTTTGCTCGGCTCTCTATTGTGGGCTTTATTTTATGTGCATTTAGGCACAATGCGCTTATTGACTTAATGACAAATGTAGTTAGGCAACACAATGACTGACAATGCACTGCGAGAAATGTATCTTTTTCGAGACTATGATAAAAAGGATACTGAGTGCCTTTTATATTGTTGCTTACCTATAGACAGCTTGATTGGCGATTTGGAGTTCATCGTTGTAATATTTAAGATCATTAACTTAAATATCCCTTATTCCGTGAAGTACAAAATATAATCTATTATTATTGTAACACAAATTCTGTTAGTGCTATTTCCCATTCCTGTTCCTGATCCCCTCCCACGTTTCCCGCTGCCGTTAACATTTCTCCTGCTGTTTGTGAGCCAAAGTGCTTGAGTTTCCTATTATAAGAGACTTAACTGAAATTGCATTGTGGCCTGCATCCTGCACTTGTCACCGCCAGCACCCCGAGCTATTTTCTTCTTCATTCTCCGCTGCTTTTCTCAAGGATGTTGACAAATTTACAAACGCGAAAGTGAATTTTCTTTCAATTTTGTTGCAACGACGGGAGCCAGGAGCTGCTATTCCGATCGCCTCTCTCTTTCGCCCTGCCTGCTGGCGTCTCTTTCACTCGAACTTTCCCTCTTCCCCGATTTTTCCTTTACTGCCTGCCTTTCCTGACAGGCAGGATGCGCGCCTAAGTAGTTTTTGTATAATTGCACTCGTCATTGTCGTCGTCGACATTCTTGCCGGGGATACTTGTGCCTGTC |
TCTCTTGCACCTTGCTGTCCCTCTCTTTCACTCCTCAGCAGGAGACTAGCTCGCGGCAGTGACAACAACAGCAATTAAGGAATGTTAACAAGCGCACTTTTCAACTTTGGCTCGCGGCTCTCTGTTGCACTTCAAAGGTGTGCCGCTGTGTTTTTCCCTGCGTATGTGTGTGTGTATGTGCCTGTGCCACGCCTATCCGCCTCCTTTCTGCGGCTTTGTCAGGACACAATCTTATGCAGACTCTTCAATTTTGTGACTGATTGGTTGGGAAATCATTCGAATAAGACACCGTGGGATATTTCGAGCTTGCTAGCTGTATAGTAAACCTCATATCAACCTTTGTCCAGTCATATACATCGAGTATTAATCAAAAACATCTCAAATATGGACAGGTCCTTTTAAATCATCATTTAAACCAATTCACTAACCCACTTCAGGTCGGTTAACCTTAGCTTCTGAATTATTCATAGAGCCACATAACCCAATTGCATTCTCAAGCGGACGATGATTGACACATAGCCCACTTAAAGTACCCTGCCAATTAAAAATCAATTTTACAGGACATATCTTGCGTGCCACCGTGTGTCTTCATTTAACAAATCTGAAAATAATTGAAAGTTAGCAGCATAGGTCCCCGAAAACTATGGCATAGTTTTAAAAATTCCCCTGTTGACAATTAAGCTAAGCAGAAAACACAACAAGGACCCTGGGAACCCGAAGCGAAACCAGTCGCAGACACAACAGAAGCAAAACAAAAGCAAACTGAAATGCGTCAAAGGCACGCAACCCTAAACACCGAAAAAGGATAAAGGAATGGGAGGTGAAAGGAGCGTGGGTGGGCTACAGATATATCCACCCACCCATGAGAATGAAGGAAAACTGGTTTCGGTTTCGGTTTCTGCTGGTTAGCAACCCTAATTTACTTTTAATAACTTCATAGGCACAGCTCAAAACAGGCAACGCAGGCAGGGGAGCATTAAAAGTGTCCTTTTTCACATGGCCGAGGAAGAGGACGAATGTGGCGGCGTTAATTTCGATTTTGCTTAACGAGGGTTAAATGCCTGCCCCACGCCAACAGTTTAACGCA |
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