CRIMIC CR00948
CG3991
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
GCACTTTTTCGCACTCTCTATACCGG |
TGCACCACCGTGACAGGAATCTCAAA |
Images
Construct Design Sequences
| sgRNA sequence |
TGGAACGTGTCGAAGACAAGAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| AAGAGTCCCTTACACAGCGC |
AGCCGACACACACCTAACTG |
CAAGAGGATTGGACTGTGTGA |
AACTGCCCTGGGACAAGAAG |
| Amplicon 5' |
Amplicon 3' |
| AAGAGTCCCTTACACAGCGCTGTGAACGCCTGGCTGGAGTCCAGTGGGATCGACGCGCACGGCAATGGAGCGGGTGCCATAGCTGAGATCCAAGAAAGCTCCACACTGCACCCACGGCTGCGAGGCAATCAGATTTAGCCGTACATTGAAGTTAAACTTGTCCTTGGGATCTGTAAAATTGGAACGGTAAGAGCTGGAATTGGGTCTCTATAGTCTCTACTCACCCGCCTCCTTGTCATTGTAGAAGATGGGCTCTATATAAACGTTGTAATCGATGGAGTTACGCTGCACGCCCTGACGCAAATGAATGCCCTTGTCTGCGTTGTTGCCCACGCGCACGGAGAACCTATAAGATTAAAACGAAAGAAATTTATTATTTGGTTGGAATTTTACAAGATTGCTTTGACAATTGTTTACTATTCGGTCATCTTAACGATATACCTACTAAATTATAAAACAAGTTAGCCCTTACTCAGCTAGTGTGAATGCGAACGCGAAATTTAATCGTTTTTTGTTTAGAGATCCTGAGATCTGGCGTTCCACAGCACTCACCTGAGCATATTATCCTTGGACTGGCGGTGCTCCGTCAAATGCTCGAATGCCTTCTCGACATTGAGCAAGCCATGGCCCTGAGCAAAGGGATCTACATAGCCCAGTTTGGTGGCAGTGACGCTGATCGCTCGCTTAATACTGTACGGCGAATACTCGATGTTCTGTTGCTTCAGACCGGAGATGAGCAGCGCCACTGCGCCGGCGACGTGAGGTGCCGCCATGCTGGTACCGTTCATCAGCTGGGACTTGCTCATAGTAAACTGTGGCACGGACGCAATGGCTCCGCCCGGAGCGCATACGGTAACGCCTTGACCTCCGTCGATGCAGGGATCTCGCGATGTCCAGGTGTACACGTTCCCGGGCAGCTTTTCTCGCATCGCATACTCGGCCTCCATCATTTGGGGTGATACGTACGCGCCCACGCCGATCAAACTGGGCTGGCTGATGTCCGGCGGAGTTCCCACAGTACAAAGTGCCGGACCATGGTTGCCGGCCGATGCCACCCACACCACGCCATACTTGTTGACAACCTCGTTCATGAGCTCCCCAATGCGGCTGCAAGAGATTAGAGATATAGATTAGGTATTTTTTCATGAATTTTGATGGTTATCAGTTAGGTGTGTGTCGGCT |
CAAGAGGATTGGACTGTGTGAATATTTGGATTTCTCATGAAATTTACCTAGACGCACACATCTTTCTTCTGCGACGCATTTCATCCGACCCAGACCCTGACCCAGACACACTAAAAGACATCACCAACTTTGGGATTAGTTCCGCCGGCCTTTCGCACTTGCTTACCCGGAATTTGACCAATTGGCATGCTCGCCATAGCTCATGTTGATCACATCGATGCGTCTGCCATCGCGACATAGTTCCATCACCTTAGTCATGGCACGCACTAGTGCCGTGCCGGTCTCCATAGACCCAAGTCGACCATCACCGATGGTCATAGAAACGATCTTAGCATTCGGCGCCACGCCATCCACGTCCCGGGAGCTATGGTTGCCGCTGGCAATAGACGATACGTGGGTGCCGTGGGGTGAGCTCATGCCGACCACCTCCAGTACGTTGCCCTCGTCGTGGACGTTTACCGATATGGAAAGAAAGTCGTCCACATTGCGGGTCTCGTGCGTGCGGGAATACTCGCCAATGCGCAGAGCCTGATCCAGATCGCCCTGCTCCGTGGTGTCGACGATTGTCAGCCATCCGTCGGCCGTGGGAAAAAGGATGCAATCGTAGGAGGTCTTAATGTCGCCGTACACCTTCTCGTAGCTATTCAACATCTCTAGCTCAAAGTCTAGATTCTCTTTCAATATCTTCTTGTCCCAGGGCAGTT |
Search Again
