CRIMIC CR00131
CG3140
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
TCAATCCGCCAGAAGCCGAAGAGTT |
AAGTCGCTCTGCGAGAGATTCGCA |
Images
Construct Design Sequences
| sgRNA sequence |
TCTTGAGGCCAGGAATGTTCTGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GCTATCCAGGCCTGAACTCC |
GAGGCCAGGAATGTTCTGGT |
GCATCTTATCTAGCCGATTGGT |
TCATCGCTGCGACGGATTAG |
| Amplicon 5' |
Amplicon 3' |
| GCTATCCAGGCCTGAACTCCGGCGTGGTCCTGCTGCTCCTAAACCGCATCCGTAACTCAAAGTCCTACCTGGAGAAGCTGACCCACTCGGAGGTGCACACCCTGGTCGCCAAGTACTCCTTCAAGGGCCACCTCGGCGACCAGGACTTCTTCACCCTGCTGGGATACGAGTACCCGAACTTAATCTACAGACTGGACTGCATTTGGAACCGCCAGCTGTGCACCTGGTGGAAGGATCACGGATACTCACAGATATTCGACGCTTATTTCCGGTGCGAGGGCAATATTAAAATGTACCATGGCAACTGTAATACGCGAATTCCAGAATAAATAAAATTGTTATAGGAATATTAAATTAATTAAATGCAGGGTATTTTTATTTGGTATAACTATTGTAATGGAACGTTATGTAATATACGGTGAATTTAATCTTAGTTTATAGACCATTATAAATGGTGATATATAAAAATGAAATTTAAAGATAAAAGACAGCCCACACCTATCACCATTTAAAATATCAAAACGATGTAAGAATGTTATCGATAGGCGAGAAGCAACAGAGTGGGAATATGCCGCACAGGGTGTTTCGTGTGAATGATTTCGCCTCTTGTTTCTTATCTTTAACCTTCATTTCTGGCTCCGTTTGGCTATGAATTAGGAGCAGTCCCGAATAATCAGTCGAGCATACGTAGTTAACGATGGCGCCGAATGCAGCAGTACCAGTGGAGCGATATGAGCCTGAGAACATCGGGATCAACGCCATTCTCCTGGGGCCGCCGGGTTCCGGCAAAGGAACGCAGGTGGGTCAAAATTACATAATGATGTGTTTTTTGTGACACGGACGCCATGTAGTCTATAAATAAATACAACGTGTTCAAGTGCGCGGTTTTTGTTTGCAAATAATCGGTGAAGCTCGTTAATTCTCTTGGAAGAGAATTAAAACTGGTGAAGTTATATAAGACGATGTGTACATGGAAATGTATATGTATGTATCTACGTGCCAAGTATGTACATATGCGTCAAGCAGTGTCTTTCTCTTGACCCTGAATCAAATCAAAGCCTTGGCGAGATGTGGCGAATTAAGTGGAACACCTTTAGGCAATCGATTGGGTATTATGCAGACCATCTATTAAAAGGCTACCAGAACATTCCTGGCCTC |
GCATCTTATCTAGCCGATTGGTTACCTTGCTTACCTTCCGATATGGTGCTAAATTGATTAATTGATTAGCTCTAACTGAAACTTATCAATGTTTTTAATTATGCCCATTTCAGGCTCCCCTGCTGAAGGAGAAGTTCTGCGTGTGCCACCTGTCGACCGGCGACATGCTGCGGGCGGAGATTTCATCCGGTTCGAAGCTGGGAGCCGAGTTGAAGAAGGTCATGGATGCCGGCAAACTGGTCTCCGACGACCTGGTCGTGGACATGATCGACTCCAACCTGGACAAGCCCGAGTGCAAGAATGGATTCCTCCTGGACGGATTCCCCAGGACTGTCGTTCAAGCTGAGAAGGTAGCCACACGTGTGAAGCACTTTGGACTTTAGGTTATAATCCGTTCGAAACGCACTTTGCAGCTCGACACATTGCTGGACAAGAGGAAAACCAACTTGGATGCTGTCATCGAGTTTGCCATCGACGACAGTCTGTTGGTGAGGCGCATCACTGGCCGCTTGATCCACCAGGCCAGCGGGCGTTCGTATCACGAAGAGTTTGCCCCTCCAAAGAAGCCAATGACGGACGATGTAAGTATCTCTATAGGACGCTATCCAAGAACTCTAAATGAAAGCAGACCTTGTGTACTTGCAGGTCACTGGAGAGCCCCTAATCCGTCGCAGCGATGA |
Search Again