CRIMIC CR00312
CG15923
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
CCGTAATTTCTTCATCTGTCCAGCGAC |
CCTCTAAGTTTCGCAGATGAATCAAACCC |
Images
Construct Design Sequences
| sgRNA sequence |
CGGATTCGGCTTGATCGACTAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| TGTTTGTACGACGTGCTCGA |
TTGCCCGTATAAGATCTGAGATC |
GACGGATTCGGCTTGATCGA |
CTCTGCTGGCGACTACTCTG |
| Amplicon 5' |
Amplicon 3' |
| TGTTTGTACGACGTGCTCGATTAGCGGACGTTGATGTGTGCTAGCTATCCTATTGCAAAACACGAGAAAAGCCAAGTAGATCATGCGGGGCATCTGCTAATGTTTGTATGATAAATGTGAAACTATGGAAATTATTTTATCTGTTTTAGCCGTAAGTTTTACGATAACTAAAGATTAACCACTGTATATGAATGTACTACTTACTATCGAGTGGATTATGAAATAATAAAAAAAAACTTCCTGTAAGGGCTCGCATATCATTTATATGGCAAAGAATCCATCCTCTCGCAGATATTGCTCCTCGTTTTGAGGCTACAAGAGGAACCTCTCGTCGATGAGGGTCTTGCGGGAGATGTTCCTTCGCACCCAATGGCGGAACACTTGGCGTAGTAACTGAAATGGATTATTTTTTAATTATTTTGTTAATCAGTTTTGAATCATTATCCTACTATGTTTAGGGCACAAGCGCCAGCAGCTGCAATTAACAAATTAGAGGTCATCAAGAAGATCGACAGCACCAAGAGGAGTTCCAGGACAAAGATGTGCAAATGGTTACCCAAGAGGAGAACGCGAGAGAAGCTGAACGAGTCGTCTATGTAAAAGGTACCTCTGTCTGAAAATTTAAATGGATTTAATACATAAAAGAGAAACTTCGAATTGGGTACTCACTTTCCGTGATGTAGTTGTCTATTTCGCAGTTCAGCAATTGTTCCACAAACGACTTTTCCTTAATAATGTAATCCATGTCCTTGATAGCCTGTCACAAATATAAAAAGAGAACATTCCATCTTAGAATAAAACTGAGCTTACAAACAGCAAAAAGAGTTGAGATTTATTAGTTACTAGGTATTCTAGACTGTAGCTCTTAATAATGCTAGAATCTGTAAGCTTAGTCTTTCGATTTTTTAGATCCTCAGATCTCAGATCTTATACGGGCAA |
GACGGATTCGGCTTGATCGACTAGGCCATATTAACTGGTTACATACTTCAATGGATCAATTAAGGTAGACTTTAAGCTTACATGGTCGATAAAGGCGCAGAAGAAGCGATTGACGCTGGCGTACGCAATGGAGGTACGTTCCATCTGTCCATCGATGCTCGGAACGATGTCCTTTTGGTAGAGCATGGTCTGCGAAAGGGTGCCGGTCAGACTGCGCTGAAAGGGAAGCAGGAGTCGCTCCAGGTAGATGCTATAGTAAAAACACTATGATAATCAATATCCTCATTGGAGTAAAGTCAAAATCTTACTGAAGGTTTCTGGGAGGCAGGATGACGTACGCCTGCTTGTCCGAGTCCATTAGCAATCCTCGTCGCCCACACATGGTCTGGGTTCTCTGCAGCTGCAAAATCATCGAGGACATATCCGTATCGGCAAAGCCGTGCGGAGATCGTCCGTGGATGTAGTAACCAAATCCTGGCTCCGTCAGTGAGAACAGAGAGATGTTGGCCAGACTGCACAGGTCGATGAACTTCTGGAGGGGATTGGCTATAAACAGTTGGTGGCCTATTATCTGAAGTAGATATGTGAGCAGATAAGCTGCCGCTATAAGGCAGCAACGTAGAAAAATGTCACCCTTGGTAAACCTCTCCAGAAACTGATAGCCAGCGATGACCACAAATACCTGACCCAAGGTGGAGTACTTCTGGGTGACACTTAGGCGAATCCAGGCATTGGCCGTGAAGAGAACTCGCCAGGCGGAGACGCTGCTCTCGGCCACAAAGGTGCGCACGCTAGAGCAGAGAGATGAGCTGTCCAGATTCAGGCGTTGTCCTTCGCAGCTGCTCCGCGGTCTTTCCCAGTCGATGAGAAACAGTTCCAGCTGGCTGGAGCGCCACAGGTGGATGCCCAGAAAGAGGAACTGCAGCACAAAGGCGGTATTGAGTAGGATTTTCAGTAGGCGAGAATTGGAGGCTTGCAGCAGTAGGAGCAGCAGAGTAGTCGCCAGCAGAG |
Search Again
