CRIMIC CR01498
CG4162
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| short band |
OK |
CACTGCAGTGTTTTCCTCGCGTGTTT |
ATGGGTCGGTTCCAGCAATCCTTGAT |
Images
Construct Design Sequences
| sgRNA sequence |
ATAATGACTTCCATATGTTGGGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GGAGTACGACCCCAAAGCAA |
TTGGGGATCCATGGAGTCCA |
ATCTGGATCCCACACCATGC |
GGCTTGGACTCCTGATCCAG |
| Amplicon 5' |
Amplicon 3' |
| GGAGTACGACCCCAAAGCAAACACACACATAAGTACGTAGTTTGGGTGCACAATGTGTGTGGGGAGAGCGGCACAATGCTGGTCTGGTCATAATATTGTCATGCCACGCCATGCCCGGCTCGCCTTATCAGGCCCATTCCACCAGTCTTTTACGTAAAGCCTCGCAGAATGCGATTCGCTGGTGAATCACAATGCTCCGCAATCTCCGAAGGCAGCGGTTTTGGGTGGGCGTTGATTTGTCGGTGATAAGAGTTTTGTTTTAAAATTCATGGACTCTCCCTGCGTCTGGGCTGCCAAAGAATAACTGTTGGCGGGTATACTTAACCGATTATCATATTTTTTATTATTCATAACTTTTTAAAAAGATAAGATAAACATATTATAACATTACATTCATTGGGTAATTCCGATACAATTCGATCTTTGGGGAGTGAGCAATTGTTAGTTTTCTTTTAAGTACCTAAAGATTTCATTATTGGTAGCTTTGTATTTCTTTTTTTCGGAAAGTAATACAATGGTTTACATAAAATTTATTTAATGAAATAGTTCCGTACCATGGCGACAACTTATTGAAATCATTCAGCTTGATTTAATGGCAACTAAAGACCTTTTCAAATTTTTAATCACACGAAAATAATGTTAGCGCGTGCAAGTCTCATATCTCCAATATCAACGGATAGAAAACTTTTATCAGCTCGGCAAGATTACAGTATGCATGATTTCAAAGGTAAAGGCTTAAATAAAAAAGAAATACTTAAGAATATGAAATTGCCAAAGCAATTATCTACTGACTCACAGCTAATACTTCTTGGAATCCCCACAAAATCGGTTAGAACTTGGCGTGCGCTGGTCGATTGTCAGTCGTTATCTTGGACTCCATGGATCCCCAA |
ATCTGGATCCCACACCATGCTCCCTACTTAGCTGTCCTTCTGTACGCGACCCATAGCGTAATTAGTATTACACTTTTTCTATTTATTTATTTATGTCGGTTTCGATTTTGATCTTTCTACGGTAGCTTCGAGCTCGTCTATATACCCTAACACTGGGACTCCATTAAATAACTAGGCATCGCGTAACCCACTTGACTTTTCGCTCCCCGCACTCCGTCTCATCGTATTTAGTAAACAAGCCCGGTGGCAAATGATAATAAACACGAAATATTATTGTCGCCGACTCGTTATTGTTATACGATTAAGCTGACGTTCAACTATATATATTTTTCGGAATATATATCTGCAGTTTGTGTTGCTCAGTTAATGAGATATATATATGTCTCGTATCGGATCCTTTGGCCAGCAAAATCAATAAGCAAATGATATATACACTTAAATAAGTACGTTAATTATAACGCTCTATTTGTTAGCTGGTGAGGTTGAAGTGTGCCAAGTTGGGCACACAAATACTATATACATTTATGGCTTCCCCTGTATGATTTTTGTGTCTGTCTCTTTTTGTGTGACTTCATGTCGAACTCGGCTCATGTGAGCGGTGGAGCAAATTGTTCAGCAGTTATCGTGTGATTGTATTTATATATGTACATACGTACCTTGCATATATGGATCTTTTAGTTAAATGTTAATTGACTGATGAATTGACTGAACGCATAGAAAGGGCAAGCAAGTGAGAATGTAGAATTTTTCATTTTTTTATAAAAGCGTTTACGCATGAGAACTATAAGTTTTGATATACATACTCGTATAAATGGGTATTACTGTGCTGATCCCTTTGAAGCGAACATGTTTACAATCTGTCTTACAATTTCCCATCTAGCCCGTCTAACAATATTGATTATTTGCTCTTTGATGAGTGATTTTCAATGGAAATTATGAAGATTCACAGTGATACCAATGATAGCGATAACCAGCCAATAATCGTTCCTCTCCCCTCTTACAGAGTCCCAGCTCGATCAACTACCATCCGGAAAAGAAGTCGATCCCCAATGGGCATGCCTCCATTCTGGATCAGGAGTCCAAGCC |
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