CRIMIC CR00325

Gene nonA

CG4211

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:16362730..16362747	14B18		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	ACATCACGAAAGGGCTTTCCTGATGC	TTCAAAGGTGGGCCCAGAAATGCTG

Images

Construct Design Sequences

sgRNA sequence	TGGCTATAAGCATTATGAAACGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAACCGGACTTTTCCAGGCA	AGCCATTCGTCGTCAATGTA	GGACCGACTTTGGCTCGTAT	TCTGCAGTGCCGTCATTTCA

Amplicon 5'	Amplicon 3'
AAACCGGACTTTTCCAGGCAACAACAATCCAAATGTAAGTTATCTAAATTCGATATAGTTGATTAGATTATCGCGGGCAATTAATTGTATACAAATATTAGACATTTTTCGGGCAACTGACCACATACATCTTTAATCAAACATATGCATGCATATAATAGCCGTTACTGATAATTGTTGCAAATATTTTATGTACAAATGCCCAAGCAAGCATATATGTGTATATACATATCGAATACTTATAAAATGAACACTTGTAGCTAAATGTGCAGGGGCAGGTACATACATACACGAGTAGAAAATATGTATGTACATATGTATAAACGAGTAAATGTTCTTGACTTTTCTAAGAGAAAAGAATTAACATTATTTATTGGGCCAATATGTATTTGTACCCACCTTAAGCTGCGTAAGTCATCAGTATTTTCCCGCTAATTAAATAATCTTTCAAAACGTTCATTTTAAACAGTAGAGTACGCATGTATTTCTATTGAACTATCACTAATTTTATGATTCCCAAGTACTTGTTTGTAATATACATATGTTTAGAGAACAGTACACATATAATTGCACATTCCTTGTTATTTGAAAAGGAAAACATTACGTTTTTATAGGAATTAGTCACTACCTACAATACATTCTGGTAAATTATAGTGCTCAGTGAAGCAATTAAATGGTTTGCTTATTGATTACATTGACGACGAATGGCT	GGACCGACTTTGGCTCGTATAGTGGACTGCGTTTCAAAGTATCGCGTGCTTGAACTACAAATACTAAAATTAATCAAATTGCTTAGCTTAGTCTATAACCTGTTTACCCTGGCACTTATAGTACAAAATGTTTCGGATAATTGGTGCCAGATAGTTTTCATAACATCCAAGGGATGGTTTTTGAATTGCCTAGAAACTACAGATGTTTTGGAAAACCGAAACTAGATTCCAATGACTTCTATCCTTTTGCTGCTAGAAGAAACTTAACTTAAGTGTACTTTGCTGAGTGGATGTATCAAGGACAAACCCGTTTTCCAAACAACTAATATTGAGTACTGCACTGTAAGGCTTAATGAATTTATATATAATCTTTAAAGGGATGAAAAGAATGTTTATCTCATTAGAACAAAGACCGTATTTAAATTCACTTCTAGTTGTCAATTTATATAGGGGAAGTTAAATAAGGGAGGCACGCCTGTAGAGCCGATTCAATCGATATCAGTTCAGTTTGTATTCAAATGGTGTAACTGAATTGAAATGACGGCACTGCAGA

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