CRIMIC CR02196
CG2616
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| short |
OK |
GGAAGAGCTAACGATGGAATGGCA |
CATGTGTGGGAAATCCCAGCACAA |
Images
Construct Design Sequences
| sgRNA sequence |
AACACAATAACAGCTAGCTGCGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| AAGGAAGACTCAGGCGCATC |
TCCATTTGCTAAACTCTGCAGC |
TCACAGAGAACTACCTGGGGA |
CCGGAGACTTTAAGCTGCGA |
| Amplicon 5' |
Amplicon 3' |
| AAGGAAGACTCAGGCGCATCCAGTGCAGAGGCGAATCCAGCTGAATCGGAAATTTCGATGGGTCGAGGACCCCGCCCAAGGTCCTTTACTGGCGGCGGAGGCGGTGGCGGAACAGGTGGATCAGGTGGAAGTGGTGGTACCTCCGGCGGCAACAATCTCAAGCCCGAAAGAAGCGCCCGGGAAGATGACGCCATCAACCGTCTGACGGACTCGAAGTCGAGTCACCGAAAGCTGCTCAGCGATCCGCGATTCCAGATTCGGCCGCTGCAGCAGGTAATCTCGGCCTGCACCGGGGCCATGATCACGGCCTGCTTCATGACGCCCCTGGACGTGATCAAGACTCGCATGCAGTCGCAGCAGTCGCCGGCCCACAAGTGCTTCTTCTATTCGAACGGGCTGATGGACCACCTCTTCGCAAGTGGACCCAATGGCTCCGAGTTGGCGAGCCTGCGGCAAAGACCACAGTTTTCCAGCTCCTGGGATGCTCTGATGAAAATCAGCCGGCACGAAGGATTGGCCGCTCTTTGGTCCGGCCTGGGTCCCACCCTCGTCTCCGCCCTGCCCTCCACCATAATCTATTTCGTGGCCTATGAACAGTTCAAGGCCAGATACCTCCAGATTTACGAGAGCCACTATAATAAGAGTCAGGAGCCCCGGCACCTTGAAATCAGGTACTTACGTAACCCTTTAAATACATAGTGTAATACATACATGTAGTTTACCTGTTGAAGCGCTCATTAAGCAATTCTTCATAGCGAATATTTACATATCCAGCGCATGAATAACCATTTAGTTTCAATAAATTGTAAAATCGTTTTAGCTTTAGATAACAAATAAGCTGCAGAGTTTAGCAAATGGA |
TCACAGAGAACTACCTGGGGAATACTAGTTTAAAAATATTTATTTTTCGTTTCATTTCGCTATTTCAAATGAAAAACATCTAAATTAAGGTTTATCAATACACATGCACATACAATACACATATTTCCACTGTATTAGACTGGATAGAAAATGTTTTGATGTATTGTACGATTTATTCGCAACTTCACAACCTATATATAAACCTATATAAGAATATGATTTAAGGATTTACAGGTTTAAACGTTTTCCATACTTCTAGGGATACCAAGAAAAGTCTACCGTCGGTGGTTCCCATGATGTCCGGGGTGACTGCTCGGATTTGTGCTGTTACCGTGGTGAGTCCCATCGAGCTGGTGAGAACCAAGATGCAGGCCCAGCGCCAGACCTACGCCCAGATGCTGCAGTTCGTCCGGAGTGTGGTGGCCCTTCAGGGCGTGTGGGGCTTGTGGCGGGGCCTGCGTCCCACGATCCTCAGGGACGTGCCCTTTTCAGGGATTTACTGGCCCATCTATGAGAGCCTAAAACAGAATCTCGGCCATGGCTCACAGCCCTCGTTCAGTCTGAGTTTCTTGGCAGGCGTGATGGCAGGGACAGTGGCCGCCATTGTGACCACTCCTTTCGACGTGGTCAAGACACATGAGCAGATTGAGTTTGGAGAAAGGGTCATCTTCACGGACTCGCCGGCGAGGGATTTTGGAAAGAAGTCGACCTTCAGCAGACTAACGGGTATTTACAGGACACACGGCGTGCGGGGACTCTTCGCAGGATGCGGACCCAGGCTTCTGAAGGTGGCACCTGCCTGCGCCATTATGATCTCCACCTTCGAGTACAGCAAGTCCTTCTTCTTCCATTACAACGTGAGGCATCATAACGAGGCCCTACTCCTGGACAACCCGAAAGACACGACGGTCGAAGATGATGACATTGAGTAGAGAGATGGGTGGCTCCTTTGTAAATAAAGAGCGCATATTTTATGTAATCCCCGGGTGGCGAGGCGAATTCAATATCCCCAACGCTTTACGAGTTACGTGTTACGTCAATAAGGCGAAGCCGGGAAATCAATTTCCATTTTCGCAGCTTAAAGTCTCCGG |
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