| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_SA-GFP-Stop_p | II:7999670..8004286 | 28D3-28D3 | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | L-F CGCTTAATTAGATTGATTCATTTGTGGC L-R aacttgtggccgttcacgtc | R-R CAATAACTCGTTGCTTTACCTTAAGACTC R-F cgcatgattatctttaacgtacgtcac |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|