CRIMIC CR00920

Gene Toll-4

CG18241

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:9085326..9085338	30A1	FBti0199686	79358

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAAAACTTTCCGCCAGACGATGGAG	CCGCAGGGCTTATTCTCATATCGACT

Images

Construct Design Sequences

sgRNA sequence	AGAGCTATTCGTGCCTTCCCCGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGCCACTTTGCCATTTCCG	AGGCACGAATAGCTCTTCTCT	GCCGGCCATAATTCATCTGG	CGATCTCACAAGCGTACCGA

Amplicon 5'	Amplicon 3'
TTGCCACTTTGCCATTTCCGGTTTTGACGGCTCGGCGGGAATTTCTATTCCTTAGCCATAATTGCAAAGTTCCCGCTCCTGCGGCTCCTTGGGGCCTCAGCTCATCCCACAGGCTGTCCGCAAACAATTCCGAGTGATTTTCAATGTGTATGTGAATGAAATTAAGCAATTCCAATTTACCTAAGGTCCTTTCCCGCATCACTGAGTTCACGTAATGATTTCGCAAATGCAATAGCAAGAAAATTGTTTGAGACTTATGGAACTCATTTGAACAACACCAACAAATAATTACTGGTTGGTTTATTAAGAGGCCTTCAAATTACGGAATTTCAGTTAACTGTAGCAAATATGAACTTGAACATCTATTTAAGAATTAAAGCCGAATTCGCTACATGCATACAACCAGACAACTTGCTATTCATAACAACACCCGTTGACAATTGATTACGCATTACTACAATTTAATTATTAACATTAATATATCAATTTGTATTTAATTCATCCTTCCTGTGAAACAGAAATTTATTTGTCATTTATTAAATCTTTAATAATTGGCACGGTCTCTGTGTCAAGCCAACAAGCCTAATTAACATTTAAAATTATCAGCTGACATAAATTGTTTGATGACCATAAATATTTGTGCCGACGAGCGGAGGCAGAAAACTTTGCAGACAAGGCAATATGAAAAGCACAAAAGGACAATAAGGGTCCCAGTTCCGATTGCCGTTTTCCCCTTGCCACAAAAACAGAAAGAGAGGGCAACGGATAGCGCAGTAAAGAGACGGCAGCGAATAAAAAACAAATATATCAAAGCGAGCAAGGGGCATAAATGTAGCGTAATTTAAATAAAATTGCATTAATATGTTCTCTCCACCGAAAGGGATCTCCAGCTGCAGCCTGTGTCCTGGGCAAATTTCCAACCAAGAGAAGAGCTATTCGTGCCT	GCCGGCCATAATTCATCTGGGAGGCAACAAAAACACACACAATCACACACCACACAACTATCGCCGGACCTCTGTTGCTCAGCAAATTCACACCAAAAATCAAAAAGCAATAATGATGCTCGAAATCTGAACAGCCGGGTCCGGGTCCGGGCTCGAGTTCGGGTTGTGCCTGGCGAGATGCGGATGGGAGGTGTGAAAAGTGGGCATGTCTGGGCCACTATAAATGCATGAGTGTGTGTTTGGGCTACGTGGCAAACTTTTCGAGTATTTGTCGTTATAAATTTGACATCAATTGCCACGGCATGTGACCAACGAAGGTCTGACAGTCCAGGAACAAGCCAGGCGGTTCCAGAGGTGGACGGACTACGGCTGAAGGAACGAAAAGGCGGTGAGGCACTGCTGAGGATGTGAGCCAAATTGCGAAAAAAAACAAGGCTCGATTTAGATATGGAAGGAGTAAATTTAAAAGGAAAAATATTTAATAAGGAGTATACTGCCAACCAAAACCCTTTTTACTCCTACAATTTTATTCGAGTAACTCGACTATCAGATACCGACTAAGTCGGTACGCTTGTGAGATCG

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