CRIMIC CR01566

Gene CG17985

CG17985

Information

Donor Location Cytology FBti Stock number
pM37-p1 2R:7651625..7655302 43E7-43E7 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GAGATCCGAATGTGATCGGAGTTC GGATCGAAGCCATCCAGTTGGTTA

Images

CR01566-10-1-A.jpg CR01566-10-1.jpg CR01566-4-2-A.jpg CR01566-4-2.jpg CR01566-9-2-A.jpg CR01566-9-2.jpg

Construct Design Sequences

sgRNA sequence CCCAGTGTTAAAGTCAGTGTTGG
sgRNA2 sequence
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACCAGCTCAGAGCCAATGAC GATTCGTCGATAGCGCTAGC TGGGAACTATCGATGTGTAGCC AAGCGCCTGAACAAGATCGA
Amplicon 5' Amplicon 3'
ACCAGCTCAGAGCCAATGACGCGGATAAAGCAAGCGTCCGTGCGGTTGGCAACGGCGCGGGCACACAGGGTCTTTCCCGTTCCCGGTGGGCCAAACAGTAGCACACCTTTGGGCGGCTCAATACCCAGATTGACGAACTTTTCCGGGTGCAGCAGTGGCGTCTCCACCACCTCGCGCAGCTTTTCGATTTGTTCCTTGCAGCCGCCCACATCGCTGTAGGTGACGTCCGGCTTGTCCTCAACCTGCATCATGGTAACCGTAGGATCAATCTTGGGAGGCAGCGGAATGTGGATCTGGTACTTGTTGCGGTCGACACCCACACGCATTCCCTCTTCTATGTCAGTAGGGGCCACCGAGTCAGCTAGGTCCACCACGAACTTGGCGAACTGCTTAACATTGATGATATACTTGGGGTCGTCGGAATCCGCGTTGATGATCTTGGTGCATCGGGCAACCTGCAGCGGTTGCTCGTTTTGCAGGATCTGCTTGTCGGCGGCCAAATCCCAGAGCGCTGGTGGCGCCAGACCCGTGTCGCTTTCCTTGATTCCAGTCAGCTCGTTCACCTGCTTCACAGCCTTTTGAATGTCCTCCTCGATGCTCTTGATGGATTTGTGATACTGGCTCTGCCCATAAGTCTTTAGAAGCTCAATGTCGCCTTCGTCGAGGGACTTGATCTCCTTGTCCTCCTTCTCATCGTGCTTCACCTTGCGCTGGTCGTCGCCCAGGTAGTCCGGCATCTTATTCGATTGCAATTGTACGGTTTTTAAGGCAAAAAATGAGTTTATGCGATGTTTTCCTTTTGTTTTACGGCGAGAATGACTGAACAATTTGGCATAGAGATGGGAGAAGCACCGCTAGCGCTATCGACGAATC TGGGAACTATCGATGTGTAGCCACTTTTAACTTCTACTGTTAAAATACAAATCTTAAACTTTTCCCAAAATTATTTTATGGTATTTCAATTGGAAATATACTAATTAGTAACTGGAGCTTAAATTTTTAACACATAAAATGTCTAAATGCTGTTATATTTCTTATCTTATCTTAGAGCATGTTGCGAATTTCCATGCATGTGTTTTAGTTCAAAATTGGTAGATATTTGAATTGAAATAGATAAGAAACTTTGTTATTTTTAAAGTACTGATATTGCTTGATTGATAAGTAAACATACATTCTGCACCCTTTACCTTACCTGTACTTTTATTTTTAAATTAAATAATATATATGTACACTCGCACTGAAATCAGCACTGACCTTTCTTCACCAACAGTGAAAGTATTTCAGAAATTTTTAAAACCGCGGCGAACTGTGGGCTTTCATTCCCAACTGCATCTTCTGTACTCACCTTCGTCATTCGGCGCACCCTGGGCTCCCTCGTAGAATTGTCCAGCAGCTCTGCTCGTCGATGGTTTCTCGTGATCCACGGCGCCTGAAGCATTTACCGCCGCCACCAGGAGTCGCTCATCCAGCATTTGCCGCGCATCCTCGAGGGGATTGCGTTCGGGAGCTGGTTCCCGCTCAATATTGTGTCTAGGACTGTTGTTCCCGCTGCGGTGGACCGCAGGAAGGGCTTCGAGGCCCCCGTTGCCCAGCAGGACGTTGTGCACTGTAACCGGAATGCGAATAACCCGATGTGCGTGGATTTCATTTTCGCGGTCGATCTTGTTCAGGCGCTT

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