CRIMIC CR01569

Gene Coq9

CG30493

Information

Donor Location Cytology FBti Stock number
pM37-p1 2R:7700188..7700197 43E11 FBti0212932 91260

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK long band GTTTCGACTCAGTGTGACCAGAGTTG CGTTTCCAAGCTGGTAATGGAACTGG

Images

CR01569-1-2-A.jpg CR01569-1-2.jpg CR01569-2-2-A.jpg CR01569-2-2.jpg CR01569-4-1-A.jpg CR01569-4-1.jpg

Construct Design Sequences

sgRNA sequence TCTGGCTCACTACATCACCGTGG
sgRNA2 sequence
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GGATTACGCCGCTTTCCAAC AGTGAGCCAGAAGGTCCTCC GTGGGACTCTTTCGGGAGC TAAACTTCCGGCATGGGACC
Amplicon 5' Amplicon 3'
GGATTACGCCGCTTTCCAACACAACGTAAGTGGCTTCCTGGTGAATGCCAAACAAACCGATTTCAATTGCCTCAACGACTTCAAGCCGCTTAAATGGCAAACGTGCGCTGTTTATACAAGCTTATTCGTCTGCAGAAGCTCCTGCTGCCGCTCAGTAAGTAGTGTGAACTCCCCAATTCACCTGTTAGCCGCGAATTCACCGCGTGTGCTCATTACGAAAGAGAGTCCGCAACCGACAATCAGCTGTTCGATCGAGTTGTGCGTGCGTGCGAAAGAAAGGGGGGAGAGAGAGAGAGAGAGAGCTAGTAAAGGGAACTGCTGACTGTCAAATGCCGGCGGCCTTAGATTTTTCGACTATTTTCAATAAAATTGTGAAATACCAGTGAAAAATTAAAGCAAATAAATAAAATGCAGGAGCAGGAGATGGAGGTGGAAGTGGGGGACCCGGCAAAGGCGTCGAATTTGCTGCGGCTCATCAAGCAGCTGCTGCTGGAAAAAGCTTACGATGGCGTGCGGATGTTGTTTCAAAGTGCCCAGGAATCGGAAAAGAACACTCGGCTGCTGCCCCACATAGCCATGGATCTATACCATGACGTGTGCTTGGAAAACATTTCCGACGAGGTGAACTCCCAGGAGCCAGAACTATTCGACTGCTCTGACGAGCTGCTCAAGCTGCTGGCCAAGTACGCCCCACTGCATGAGCTTATGCTGGAACTGATGGAGCGCTTGGAAGAGAGTAGCAGTATCAATGTGTTCGGCGCCTATCTGCGGGCCCTACAAGTTGTGCTGCAGCGACAGGGACGCGACAAGCCGCAGGCCGTTGAGTGGAGCCTGAATAGCATCTTCACCCATCTCAAGGATTTGCCCCTGCCCGAATACCTCTCCGAGGGCTATGACGAGGCCCAAGCGCGACTCATAGAGCAAAACGAACAGGTGGAGGACCTTCTGGCTCACT GTGGGACTCTTTCGGGAGCCGCTCCGCGATGAAATTCTGCAGCAGGATGTGCGGACACCCAGCCAAATATTCAGGGATTGCGGCGTCAACCGGCGCAACATATTCACTTGCTTCTTCATTCAGCTGTTGGGACGTCCGTTGGCCCTGCTGGAGATGAATCACGCCAAGGAGGACCTTACGAGATCCTACGTGCAGCAGGTTACGGAAAGCCTGACTCAGGATGCCAACCAGTTCCTGGGGGATCCCTTCTACCTGCTCAACCTCGTGGAACAGCGTGCCAGATGGCTAAGGAAACTAGATGCCTCGAGAGGCGTCTACGAGATGAGTTGCCAGAATGTTTTCCTCATCGAGGAGAAACTTCCGCTGCATGCTGTAGCCATGTACTATCACTCGCTGTTCGTGGGTAACCAACTGCCCACAAACGCACCAAAGGTATATGCACCTTTATTCCTATTCGAAACGATTGTCTATCTGGCTGAAGTGCTACTCAGGCAGCAGGAGCCTCCCCTACAATATTGTGGTCTTCGATTAGTGGAACATCATTTGAGTACCTTGCAGCACTCAGTTCCCACCAGCTCCCTCCAGCTGGATGTCCACAAGCGCTTTTGCGAAGCCCTTTGCAAGATTGTGGGCTATTCGCCGCAGGTCGCACTTCGGCAGTTGGGAATTCATGTGCTCAGGCAATTTGTTCTGGCCTTCGATGATCATGGAAAGTATCTCATCCTTAAAAATCTGATGGGGACATTGCAGCACGGCGGACTAATGGGCTACCTCGGAGGGATGTACAAGGACCTTGTTGACAAGGCGCTAGGGCAATCTGGTCCCATGCCGGAAGTTTA

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