CRIMIC CR01576
CG10592
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
ACTATAGAAGCTTCGCTTGCCTCCAC |
TGGAGCTGATGATTTCACCATCGTGC |
Images
Construct Design Sequences
| sgRNA sequence |
GCTGTAGATAAGCTGGGTCGCGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GCTTGTTGCTATCGCCCATG |
TTGAAAAGCTCTCTCGGCTT |
GGATTCGTTCGGCAGTTCAC |
CAACTCCTCGCCTCCAATGT |
| Amplicon 5' |
Amplicon 3' |
| GCTTGTTGCTATCGCCCATGAAAGCACGTGTGGCCGCGATCGTGTGAACGGACATGCCATCGCCCAAAAATAGGATCACGTTCTTGGCGCGATTCTCGTTGAGCTTTTTATGGCCGGCCAGCTTATCGGCCAGAATCGATTGGGCCTTATCGTGCCAGAATCGTGTGTCCAGCTCCTCGTCGAGGGCCTCGAAGTCGCGTGATGATTGCAGACGGGGATGTTGGGCTGTAAAGTACGGACTTTGGGTTAGCAACTAATCGGCGGGAAAATCGGTCTAATAAAACTACTTCTTGAAGTGGTAAACAAGTTTTCAGCCTATCTCGAGCAGATCTATAAATCAGTTTTAATTATAAGCATTTATGGCCATTTGGCTGGATTTCCTATCGCTGGAAATTCACATTTGATTGAGGCTTAATAAAACGTGTTGAAAACGTTTTAGCTTATTATGGTGCTTTGTCATCATTACACTTTCGTTTGAAATACTTAGACATATACTTTGAGGAAATGTTTTCTTTCTGTACTGTACAAGCTTAGAAAAAACGCCTAAAATTAAATTGATTCTTGTTTAGTTCATAATTATTATAGCTTATTATTAATAAATAATAATTATACCCATTTGAAGCTTATAGTTAGAATCGTTAGTAACACTTGATTTAATCTAAGAATTTAGCCATTTTTGTTAAACATAAATCTATTTATAGGAATGTCACCCTATCTTTAATTTGCCTTCACGTGAACAATTGAGTCGTAAACTATTATATAGCTAGTCGATGACTGAGTGCTGTTTAAAATAGATCTAGATTAGCCAAGTCTAATTGGCCATCAAGATCTCTCTACGATAAGCCGAGAGAGCTTTTCAA |
GGATTCGTTCGGCAGTTCACCACGCAATGCCGTTCGGTTCCACTTACCGTGCTCTTGGTCATCGGCTAGATCACTCCGGGCCTGGATGACCAGGAGAGCGAAGAAGCACAAGATCAGGCAACTCTTTACCATTTTGGGGTAGGGGCGGTAACTTTAAACTGAGGAATGCTCCGTTTGGGAGTAACCGCGAGACTGGCCAAAACTGACGAAATGGCCCGGTTATATAGCCTCCGATTTATCGAAGCGGTCTTCCTGTAATATACATACGCGTTATGGGTGATTGTTTTTGTGATTTGAGCAATCTAAAGTGGCGATCGATTAATTATCTCGAGTGCCTAAAAACGTGAGTATCACGGTCTGGCTAAGAAGGTGTCGTAAATTACCCGATCCCCACCAAACTGCCCTATCGACTCCTCACACGAGCTGCACCTGATGGTATATTAATGGGTCGCATGGAACTACGGAGTACAGTATTCACTTGGCTGGCACGGTGAAAGTATCTCGAAAATGCTGAGTCTTAAGTTCTGGATTCGCCTCGGTCTGCTGACCGTCGTTTGGGGTGCAGCGGTTCCTGGAAGAGCCCCCGTGGACGAGGAGCGAATGCATCCACATCTGCCCACTAGTCCCAGGCTGCGTACGATTTCCGGCGAGGAAACCCAGGAGTTCTGGCACTCGGCCAGCAAAAAACTCATCCGCGAAAAGTTGGAGTTCGTGAGGAACACCAAGAAGGCCAAGAACATTATACTGTTTTTGGGCGATGGCATGGGACTGGCTACTCTGGCTGCTGCCAGGAGTTACATTGGAGGCGAGGAGTTG |
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