CRIMIC CR02127
CG31064
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| long band (weak) |
OK |
TTGCCAAGCACGCTCCTACTTTGTAG |
CACCAATCTGTTTGGCAACTACCGCA |
Images
Construct Design Sequences
| sgRNA sequence |
ACTCGGCGGTACTACTACTCCGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| CAGTGCAAAACGACGCTCAA |
AGTAGTACCGCCGAGTAGCA |
GTCGCTCCACCTCACAAAGA |
AAAAGTCGTTTCCCAGCTGG |
| Amplicon 5' |
Amplicon 3' |
| CAGTGCAAAACGACGCTCAATACGAACGGAAATGGGTGGCGAGCTCCGGACAAGGATATATTGTCGCAGTTCAAGGTACGAAAGATCGAACCCGTCAAGCTGCCCAAGAGCTGCTATTCCCAGGAGGATGCCTCCAGCTGTGCTCAACTGGATCAAACATGCATTTCCCAAGAGCCGGATCAAGGGAGCATGATTGTGAGCCTGCCTCGACTGGAGACGCTCGATGAGAATGGCTATATGGGCAACATATACTTGCCCTTTCGAAAGCGACGCATCTACAATCTGCGTTCTCCCAAATCGGAATTTGTCGTGGTGAAGTTCTTCCAAACCCTAACCAACTGTCATCGGTTTCAGGGCATTAGCCAGGCTAATTACAATCGCCAGTTGCTCGCCTGGATAAAGGAAAATCTGGAAATGCACTATGGCGATGATGTATTCTACCCGGGTCTGGGCGGTATTCTGCAGATTCAGGAGAGGCTGTCGAAAGAAGCGAAGAATGCGGAGGAGGAGGCCCGGGCGCCAACCAATAAGAATGACGGGCAGGGATCACCATCCTGTCCCAAAGAGGACTCCCGCAAGATGAGTCGCTCCGCTATTCACTGGCAGGAGGCTTCAGGGCCAGTCGAGGAGTCGGGGCCGGAGCCAATGCACCAGGACGAATGGCTTACCGGATTGGATAAGGTTCAGAGTCGGCCGGAGGAAGTGGAAGAGTGCCGCGAGTGCGACAACGATGAGACACTGATCTGGTGCATTGCCGCCTTTCTGACCCTGCTTCTGCTCCTCATTCTGCTCATCATTTGCTGTTGCATGCGAAGGGGCAGGAAGAAGAGGGTTGCCCCAACGGATGCAGAGGCTCCTCCGCCGAAAGAGAGGCCAAGAAAAGAAAAGGAGCCGCTGAAAGCCAAAGAACCAGAGAAGGAAAAAGAAAAAAAAGATGAAAAGGACGAAAGTGATGCTACTCGGCGGTACTACT |
GTCGCTCCACCTCACAAAGATCGGTGCCGAGCAATCGTAAGTCAAATAAGTTTGTGGCAACTAAATCAACTAAATCTAACTGCTCGAGTTTATAGGATATGCGAATAATCCCGAGCTAGATTCCTTCACCTCATCTTCGTCGCTGGGATGTCAGTTCAACAGGAAGTGCATGCCACTGAAGTTCTCCCGAGCGAAAGGAGGGGACTACTACGCACCGGACAAGAGCCGAGATGCGGTGGGAAAGCAGGTCACATCCCTCAAACTTCTTTCCGATGATTCGTCTCCTAGCCGAGTTCCCTACAGGGACGTTTGTAAGCCCGCTCTAAAGTCGACCCTATCCGTCATTCCCTCGTCGGACAGCGAAGTGGACAGCAGAGAGGATAGACATCGACATAGGCATGCCCGCGAAGAAGACAGGCGGAAGAAGCATAAGGACTTTGAGACGGGTCGTAAGAAAGAGGTAAGTTATCAGTGCTTCGCGTGCTTTTGATATCCTGAACTCAGAGTCACCAGCAGACCAAATCCTCAGAAGACAAAAAGCAGCCATCACCAGAAAGGCGATTGTCCAAACAAGTGAGGATAGGTAAGTGCATGACTGGTCATTTCTTAAGCCTAATCGATTCATTCACTTAATCCTCTGATGCTGCCATCTTAAAAGCAACACCGGAAATGTACAAGGATACATCCGATGATCCTCTTCTGCACAGGAAGAAGTCGCAGGAGAAACCAGATGTCCCTGCCGGTCGACAGAAGGGCGGTGCAGAGGTGAGTGGGATGTGCAAAATGTTATATGCCAAAATAATGATCTTGACGGTTTTTCAGTATTTAAAGGACTCTCCCAGCTGGGAAACGACTTTT |
Search Again
