CRIMIC CR01435
CG30008
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
|
|
Images
Construct Design Sequences
| sgRNA sequence |
ACTTGACATTAGCAATTCAGCGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| AACTCTTGGCAAGCAAAGCG |
TCCACTGACCCACATGCAAA |
CGGGTTCGCTGGCTTACTTA |
GTCACGGGAGCTGGATCAAA |
| Amplicon 5' |
Amplicon 3' |
| AACTCTTGGCAAGCAAAGCGGATAGGCCATCATGCTTGCTTTATATCTTTTGGGACGAAAACGCGAATTGCAAAAAGAAACGTGCTGCCCTCTTGATCTACATAGTTTTTGCTTACGTATACCACTAGACGTCATAAAAAATCAATGCAAGTGTGGAATTTAAAATGAGTTGGTTCTTCAGTTCATAAAACAAACAACGTCGTACTTAAACTATTACGGTGAATTATGAACTATTAGTGAAACTGCAAAACATTGATCAAGCTTTCCTTAGACAATCTTTATTTCCATTTACTAAGGAGACACTTACCTGAAAGTGGAAGGTCTTATGGTCCACAGTGATTGTGAATGTGTTGTCCTCCTGGTCGTCGATTCCGATTAGCGCATCCTTTAAACGAACACAACCGCGCCTCACGCCCTTTATCATCTTATCCTTCGACTGTAACAGAAATGCAGTCGGAATTAGGAAAGTTCATTAAATCGCAGCTCATACTGTCAAAGGGCTATTACGCCTGCTATAAATTAATGTATTCTAATCAATGCTTGGGGAATATAAAAACAGTGTTCGGCGTGCTGTTCACGCTTAAATGTTCGCCAGCGGTCAAGGCGGTAGAGCTGCAGTCCACCAGTCCCACGCCACCTAAAGGATACAGGCCAGCGGAAGCTCAACACAAAGAAGCGAAATGGAGGCGTCAGCAAGTGAGCTCCTCTTGGATTTGTCAAAAAACTGTCTGATGGTTTCAATAAAATTGTAGGTATAAATTTGCAGCCGGTGGTGAACGTTCCTACGATTTATAAGGATCCCTGGTACATGATCACAGTGCTGGTCCTCTATCTGTACTTCGTCACCAAAGCGGGTCCGCAGTAAGTTCACATGCTAATGTCAGCGTCGCTTGTTTGCATGTGGGTCAGTGGA |
CGGGTTCGCTGGCTTACTTATATAAGAAAGAAGACCTTTAATACCTCGCTTATAGTTTCATGGAATGGCGCAAACCTTACGAGCTCAAGCGACTTATCTTGCTACATAATTTCATCCAGGTGGTTTCCTGCATATATGCTATCAAAGAGGTGGATATTTATAAATTGTACAGCAGCAGCAGCATCGGCTAATTGCTTTTCTCTCGCAGGTCCTGTACATTACGGACAACACCATATACATCTTTTGGAAGTGCCGCGACATCGGGAGCAGCCCAGAGCTCGTGAGGCGGTACTACAACTTGGCCTACTTTCTCTTCTGGCTGAAGATCTCTGAGCTGATTGAAACCGTGATCTTTGTGCTTCGCAAGAAACAAAACCAGGTGTCCAAGCTGCACATCTTTCATCACTTCTCCACGGTAACGCTGGTCTACGCACTGATCAACTTCAACGAAAATGGTAAGCTAGCCGGCTGCTGGGTGACCGACTATTTTCATAAATGCGTTCAATTGTACAGGTTCTGCTGCTTACTTCTGCGTGTTCTTGAATTCTATCGTTCACGTTATCATGTACTCGTACTACTTTGTGGCGGCAGTGGCGGATAAGACCTTGGTGCAGGCCCTAACTCCAGTGAAGAAGTGCATCACAGTGATTCAGATGACGCAGTTCGTTCTTATCCTCACGCAGGTTGCTTTCCAGCTGGTATTGTGCGGCATGCCGCCCTTAGTGCTTCTCTACTTCACCACCGTCATTCTTGGCATGTTCTACGGCTTTTACGACTTCTATAATAGTGCCTATCAAGCCTCGCAGCGGCGCAAGAGTCAGACCCCTCAGTCAGACTCAAAAAAGTAATCTCCTGTCAACAAATCACGCAATAAAGGGGTACCAACAAGTGTGTATGTGTATCCAAATGTATCTATAGCAGATAAGATAAGGCTGCGGGCCATGAGGAGTTCGTTCTGGGTGCGATATGGGTTTCAGTCAGGAGGTGCACTGCAGCCACTCACCGTGTAATAGGACAGCAGTCCAGCATTCTCGTCCAGAACGAAGAAGCGGTACTGCCAGCCCTTCATCACATTCGTCCACTTGCTTAGCGTGCCCTCCAATGTGCCAGAGCCACCACTCCCGCTCTGGGGGGGTCCCAGGCTGGGGGCACTTCCAGTGGTGCTGGCCATTTGATCCAGCTCCCGTGAC |
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