| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_PM37_p0 | 3L:4485159..4490963 | 64B8 | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| short band | no band | GAATCGCAAAACGCGTTGTTGC | ATGACTGCTGCCAGCTGTTTTCTG |
| sgRNA sequence | CCGATATGCAGCTTGCAGCTCGG |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|