CRIMIC CR00949
CG5522
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
CTTTCAATTTGTAGAGCCTCGGCAGC |
GCACAGTTCGAAGGTGTTCTTGTGCT |
Images
Construct Design Sequences
| sgRNA sequence |
AATTCTTGCGCAGTGATTCGAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| AGGACAACTGGGCGAATCTG |
CAGTGATTCGAGGTGGTGGA |
ATCACTGCGCAAGAATTGGA |
GTGGTTGTGCGTTGCAATCT |
| Amplicon 5' |
Amplicon 3' |
| AGGACAACTGGGCGAATCTGCGCTCCTACCTCGAGAGCCTCCGCCTGCCCTGCATCCCCTATCTGGGTCTCTTTCTCACCGATCTGATCTACATCGATTTGGCCCACCCACATAAGGGCGGATTGGAGCCAGAGCAGCGTCGCAATAAGATGAACAATATATTGCGTGTAATTTCGAACTATCAACAGTCGAACTATAAGCATTTGCAGAAGCATGAGGCGACACAAAAGTATTTGACTTCGATAAGGTATATAGAGGAGTTGCAGAACATATTCGAGGAGGATCAGTACAAGTAAGATTAATCTTTTGAATCCGAGCTGTTTTCTTATCTAAAAATAATGCTGTTATAATACTCATTAACCTTTCAGGCGCTCTCTAAACCTGGAACCTGCGTCGCCCTCTGGACCCAGTTCTTCGTCCTGCAGCTCAAAAGAGTCCTTCAATGTGGAAGTAGTTACTCCGGCCCTTGGATGCCTAAATCTCTCGCCGGCCAAAACCATTGGCTCCATGCGAATGGCAAGCGGCACAAAATTTGTGCCAGGCCATCGCAAGTGTCGCAGTCTGGGCACCAAGTAAGTAAAAGGAATCCTACGTTTTGAGAGCCAACACTTGGCTTCCCTTGTTAACCACCTGTTTGAAATCCACCACCTCGAATCACTG |
ATCACTGCGCAAGAATTGGACATTTGTACACTGGGTCCTTATTAATCGTTTAAATCTCTATGTCTCTCACTTCTACCGTTTTGCTTTTGTCTTATGCTTTCGTTTTGTGTGTGTGTTCGTTAATTGTCAAACATCCGCCTCTGCCCAAAACGAATGAAGATTTCGCAGCAGCAGCCTGCCGCGAAACTTTGCCGAGAAGTGCCAATGTTGCATCGTCATGATTGCGCCGGGCATCGGCACCATCTCCAACAAGCGTTGCAGATGCCGTCGGTGAGTCCAGAACAGAGTCCTGTAGCTAGGATATGTGTGTATTGCCAGCTTGATACTGAATACTACTGATTCGATGCGTTATCCTCATTTTAACATTTGATGTGTTCAGTTATCGTCTCCTCATTTTCTTTTCTTTTGCACGGACCCGAGTTGAAAATGCTATGATATACACAAATACACGACCCAACACTTGACTCATTTCATTTGATTTTGATTTGGGAAACCATGCATTACTCACCAACCTTACGCACACAACTTCCCCGAACATATTAGAAGGTTCTATCGCATTTATTTTAAACAAGAAACCTTAGTCAATTCTATGCTGAGAATAAATCGATATAACCTACAAATACGAATCGCAGTTCACCCTTGTTTGCCTTTTTGCTATTTCATTTCTTCTCATTTGTATGAACAGCATGAATGGCTCCAACCCGCAAGAATATCTTGACTAAACCATTTTCTCCTTTTACAGCATTTTTGGCAAGATTGCAACGCACAACCAC |
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